Henkemeyer M, Orioli D, Henderson J T, Saxton T M, Roder J, Pawson T, Klein R
Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Cell. 1996 Jul 12;86(1):35-46. doi: 10.1016/s0092-8674(00)80075-6.
Eph family receptor tyrosine kinases have been proposed to control axon guidance and fasciculation. To address the biological functions of the Eph family member Nuk, two mutations in the mouse germline have been generated: a protein null allele (Nuk1) and an allele that encodes a Nuk-beta gal fusion receptor lacking the tyrosine kinase and C-terminal domains (Nuk(lacZ)). In Nuk1 homozygous brains, the majority of axons forming the posterior tract of the anterior commissure migrate aberrantly to the floor of the brain, resulting in a failure of cortical neurons to link the two temporal lobes. These results indicate that Nuk, a receptor that binds transmembrane ligands, plays a critical and unique role in the pathfinding of specific axons in the mammalian central nervous system.
有人提出Eph家族受体酪氨酸激酶可控制轴突导向和束状化。为了研究Eph家族成员Nuk的生物学功能，已在小鼠种系中产生了两种突变：一种是蛋白质缺失等位基因（Nuk1），另一种是编码缺少酪氨酸激酶和C末端结构域的Nuk-β半乳糖融合受体的等位基因（Nuk(lacZ)）。在Nuk1纯合子脑中，形成前连合后束的大多数轴突异常迁移至脑底部，导致皮质神经元无法连接两个颞叶。这些结果表明，Nuk这种结合跨膜配体的受体，在哺乳动物中枢神经系统中特定轴突的路径寻找中发挥着关键且独特的作用。
