Henkemeyer M, Marengere L E, McGlade J, Olivier J P, Conlon R A, Holmyard D P, Letwin K, Pawson T
Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Oncogene. 1994 Apr;9(4):1001-14.
Neural kinase (Nuk) encodes a murine receptor-like tyrosine kinase belonging to the Eph/Elk/Eck family. Protein localization studies indicate that during early embryogenesis Nuk is confined to the developing nervous system, where it marks segments along the axis of the neural tube in the hindbrain (rhombomeres r2, r3 and r5) and specific morphological bulges of the midbrain and forebrain. Subcellular localization of Nuk indicates that this receptor is concentrated at sites of cell-cell contact, often involving migrating neuronal cells or their extensions. Most notably, high levels of Nuk protein are found within initial axon outgrowths and associated nerve fibers. The axonal localization of Nuk is transient and is not detected after migrations have ceased, suggesting a role for this tyrosine kinase during the early pathfinding and/or fasciculation stages of axonogenesis. The subcellular localization of Nuk, as well as the presence of fibronectin type III and immunoglobulin-like adhesive domains on the extracellular region, suggest this receptor tyrosine kinase may function to regulate specific cell-cell interactions during early development of the murine nervous system.
神经激酶（Nuk）编码一种属于Eph/Elk/Eck家族的小鼠受体样酪氨酸激酶。蛋白质定位研究表明，在胚胎早期发育过程中，Nuk局限于发育中的神经系统，它标记了后脑（菱脑节r2、r3和r5）沿神经管轴的节段以及中脑和前脑的特定形态隆起。Nuk的亚细胞定位表明，该受体集中在细胞间接触部位，通常涉及迁移的神经元细胞或其突起。最值得注意的是，在初始轴突生长和相关神经纤维中发现高水平的Nuk蛋白。Nuk的轴突定位是短暂的，迁移停止后就检测不到了，这表明这种酪氨酸激酶在轴突发生的早期路径寻找和/或成束阶段发挥作用。Nuk的亚细胞定位，以及细胞外区域存在的III型纤连蛋白和免疫球蛋白样粘附结构域，表明这种受体酪氨酸激酶可能在小鼠神经系统早期发育过程中调节特定的细胞间相互作用。
