Ameer B, Weintraub R A, Johnson J V, Yost R A, Rouseff R L
Food Science and Human Nutrition Department, University of Florida, Gainesville.
Clin Pharmacol Ther. 1996 Jul;60(1):34-40. doi: 10.1016/S0009-9236(96)90164-2.
Disposition of citrus flavonoids was evaluated after single oral doses of pure compounds (500 mg naringin and 500 mg hesperidin) and after multiple doses of combined grapefruit juice and orange juice and of once-daily grapefruit. Cumulative urinary recovery indicated low bioavailability ( < 25%) of naringin and hesperidin. The aglycones naringenin and hesperitin were detected in urine and plasma by positive chemical ionization-collisionally activated dissociation tandem mass spectrometry (PCI-CAD MS/MS). After juice administration, PCI-CAD MS/MS detected naringenin, hesperitin, and four related flavanones, tentatively identified as monomethoxy and dimethoxy derivatives. These methoxyflavanones appear to be absorbed from juice. Absorbed citrus flavanones may undergo glucuronidation before urinary excretion.
在单次口服纯化合物（500毫克柚皮苷和500毫克橙皮苷）后，以及多次服用混合葡萄柚汁和橙汁以及每日一次葡萄柚后，对柑橘类黄酮的处置情况进行了评估。累积尿回收率表明柚皮苷和橙皮苷的生物利用度较低（<25%）。通过正化学电离-碰撞激活解离串联质谱法（PCI-CAD MS/MS）在尿液和血浆中检测到了苷元柚皮素和橙皮素。在饮用果汁后，PCI-CAD MS/MS检测到了柚皮素、橙皮素以及四种相关的黄烷酮，初步鉴定为单甲氧基和二甲氧基衍生物。这些甲氧基黄烷酮似乎是从果汁中吸收的。吸收的柑橘类黄烷酮在经尿液排泄前可能会发生葡萄糖醛酸化。
