Pidlich J, Renner F, Ellinger A, Hüttinger M, Pavelka M, Gangl A
Department of Gastroenterology, University of Vienna, Medical School, Austria.
Dig Dis Sci. 1996 Jul;41(7):1445-51. doi: 10.1007/BF02088571.
The hydrophobic nonionic detergent Pluronic L-81 has been shown to lower plasma very-low- and low-density lipoprotein cholesterol, thus preventing diet-induced atherogenesis. The major effect of this agent is a pronounced interference with intestinal lipid metabolism. For studying mesenteric lymph lipoproteins during detergent exposure, a combined micromorphological and biochemical assessment of mucosa and lymph during steady-state lipid absorption was performed. Pluronic L-81 was infused intraduodenally at a constant rate in combination with mixed micellar solutions or saline in mesenteric lymph fistula rats. Pluronic L-81 impairs transepithelial lipid flux during fat absorption, trapping export lipids within the enterocytes and leading to a cytosolic and endoplasmic reticulum lipid accumulation sparing the Golgi region. Pluronic L-81 markedly (P < 0.001) reduces mesenteric triglyceride, phospholipid, and total cholesterol secretion almost exclusively by a reduction of chylomicron formation. Chylomicron and very-low-density lipoprotein lipid composition was only insignificantly altered, except for somewhat higher phospholipid/triglyceride ratios. The chylomicron apoprotein pattern was almost unaffected. Thus, chylomicron formation decreased dramatically without major compositional alterations. The reduction of lipid and apoprotein secretion without particle augmentation is not in favour of a selective interference of Pluronic L-81 with intestinal apoprotein B-48 secretion.
疏水性非离子洗涤剂普朗尼克L-81已被证明可降低血浆极低密度脂蛋白和低密度脂蛋白胆固醇水平，从而预防饮食诱导的动脉粥样硬化。该药物的主要作用是显著干扰肠道脂质代谢。为了研究洗涤剂暴露期间的肠系膜淋巴脂蛋白，在稳态脂质吸收过程中对黏膜和淋巴进行了微观形态学和生化联合评估。在肠系膜淋巴瘘大鼠中，以恒定速率十二指肠内注入普朗尼克L-81，并联合混合微胶粒溶液或生理盐水。普朗尼克L-81在脂肪吸收过程中损害跨上皮脂质通量，将输出脂质捕获在肠细胞内，导致胞质和内质网脂质蓄积，而高尔基体区域不受影响。普朗尼克L-81几乎完全通过减少乳糜微粒形成，显著（P<0.001）降低肠系膜甘油三酯、磷脂和总胆固醇分泌。乳糜微粒和极低密度脂蛋白的脂质组成仅略有改变，只是磷脂/甘油三酯比值略高。乳糜微粒载脂蛋白模式几乎未受影响。因此，乳糜微粒形成显著减少，而主要组成没有改变。脂质和载脂蛋白分泌减少而颗粒没有增加，这不利于普朗尼克L-81对肠道载脂蛋白B-48分泌的选择性干扰。
