Toba K, Kishi K, Koike T, Winton E F, Takahashi H, Nagai K, Maruyama S, Furukawa T, Hashimoto S, Masuko M, Uesugi Y, Kuroha T, Tsukada N, Shibata A
The First Department of Internal Medicine, Niigata University School of Medicine, Japan.
Exp Hematol. 1996 Jul;24(8):894-901.
Using 7-amino-actinomycin-D/pyronin Y (7AAD/PY), we analyzed the surface phenotypes and cell cycle of 22 hematopoietic cell lines based on their cellular DNA/RNA content. Populations of G1a, G1b, S, and G2M, the DNA index (DI), and the RNA index of S phase (SRI) were calculated by means of DNA/RNA dot plots. Two new parameters were extracted from the cell-cycle profiles: the nucleic acid index of S phase (NI) and the coefficient of variations in the RNA at S phase (SVC). DNA/RNA dot plots of cell lines revealed four characteristic profiles of the cell cycle, defined with the calculated NI and SCV. These were type 0 (small NI, large SCV), type I (small NI, small SCV), type II (large NI, small SCV), and type III (large NI, large SCV). Type O included four stem cell lines: one t(1;19) leukemia, two Ph1+ acute lymphocytic leukemia (ALL), and one biphenotypic crisis of chronic granulocytic leukemia (CGL). Type I included five ALL cell lines: three T-ALL and two common B-ALL. Type II contained 10 myeloid cell lines: five AML and five myeloid crisis of CGL. Type III contained three relatively immature lymphoma cell lines: two Burkitt's lymphoma and one follicular center lymphoma. Calculated NI/SCV (%) were as follows: type 0, 2.27 +/- 0.19/16.7 +/- 3.7; type I, 2.20 +/- 0.30/11.1 +/- 0.7; type II, 3.64 +/- 0.52/11.8 +/- 1.0; and type III, 3.60 +/- 0.53/17.5 +/- 1.9. Cell-cycle analysis of blasts using 7AAD/PY combined with surface phenotyping may yield important information for classifying hematopoietic malignancy within 2 hours of patient admission.
我们使用7-氨基放线菌素-D/派若宁Y（7AAD/PY），根据细胞DNA/RNA含量分析了22种造血细胞系的表面表型和细胞周期。通过DNA/RNA点图计算G1a、G1b、S和G2M期的细胞群体、DNA指数（DI）以及S期的RNA指数（SRI）。从细胞周期图谱中提取了两个新参数：S期核酸指数（NI）和S期RNA变异系数（SVC）。细胞系的DNA/RNA点图显示了细胞周期的四种特征图谱，根据计算出的NI和SCV进行定义。这些图谱分别为0型（NI小，SVC大）、I型（NI小，SVC小）、II型（NI大，SVC小）和III型（NI大，SVC大）。0型包括四种干细胞系：一种t(1;19)白血病、两种Ph1+急性淋巴细胞白血病（ALL）和一种慢性粒细胞白血病（CGL）双表型危象。I型包括五种ALL细胞系：三种T-ALL和两种普通B-ALL。II型包含10种髓系细胞系：五种急性髓系白血病（AML）和五种CGL髓系危象。III型包含三种相对不成熟的淋巴瘤细胞系：两种伯基特淋巴瘤和一种滤泡中心淋巴瘤。计算出的NI/SCV（%）如下：0型，2.27±0.19/16.7±3.7；I型，2.20±0.30/11.1±0.7；II型，3.64±0.52/11.8±1.0；III型，3.60±0.53/17.5±1.9。使用7AAD/PY结合表面表型分析对原始细胞进行细胞周期分析，可能在患者入院2小时内为造血系统恶性肿瘤的分类提供重要信息。
