Tagawa Y, Yokota M, Hara S, Ishikawa H, Miyashita K, Yamaoka N, Tomita M
Gan To Kagaku Ryoho. 1987 May;14(5 Pt 1):1332-7.
For the purpose of reasonable evaluation of the cell kinetics of RNA synthesis inhibitors, the flow cytometric technique using acridine orange staining for simultaneous measurement of cellular DNA and RNA content was applied in order to analyze the effects of actinomycin D (ACT-D) on cultured cancer cells (PC1). ACT-D 0.05 microgram/ml, a dose that produced little inhibitory effect on the growth curve, led to G1 accumulation after the appearance of G2M, and a decrease in RNA content was concomitantly observed at 48-72h, although the RNA level had nearly recovered to normal at 120 h. On the other hand, ACT-D 0.1 microgram/ml, a dose that produced a significant inhibitory effect, resulted in the initiation of G1-phase accumulation, a maximal peak of G2M accumulation and a decrease in RNA content at 48 h, after which there was a tendency toward G1-phase accumulation and a marked decrease in RNA content which continued for 120 h. Such a marked decrease in RNA content coincided with high values of the G1a X G1b ratio in the G1 phase. These results suggest that ACT-D has an inhibitory action on the cell cycle between G1a and G1b, accompanying a marked decrease in RNA content.
为合理评估RNA合成抑制剂的细胞动力学，应用吖啶橙染色的流式细胞术同时测量细胞DNA和RNA含量，以分析放线菌素D（ACT-D）对培养癌细胞（PC1）的影响。0.05微克/毫升的ACT-D对生长曲线几乎没有抑制作用，在G2M期出现后导致G1期积累，并且在48 - 72小时观察到RNA含量同时下降，尽管在120小时时RNA水平已几乎恢复正常。另一方面，0.1微克/毫升的ACT-D产生显著抑制作用，导致G1期积累开始，G2M期积累达到最大峰值，且在48小时时RNA含量下降，此后有向G1期积累的趋势，RNA含量显著下降并持续120小时。RNA含量的这种显著下降与G1期G1a与G1b比值的高值一致。这些结果表明，ACT-D对G1a和G1b之间的细胞周期具有抑制作用，同时伴随着RNA含量的显著下降。
