Mansour R T, Aboulghar M A, Serour G I, Tawab N A, Amin Y, Sattar M A
The Egyptian IVF-ET Center, Cairo, Egypt.
Fertil Steril. 1996 Aug;66(2):256-9.
To compare the effect of cytoplasmic aspiration versus no aspiration before intracytoplasmic sperm injection (ICSI) on the rate of oocyte damage, fertilization rate, and embryo quality.
A randomized prospective study on sibling oocytes.
The Egyptian IVF-ET Center, Cairo, Egypt.
Fifty-eight patients who were infertile due to male factor who underwent 60 ICSI cycles.
Intracytoplasmic sperm injection was performed on randomly allocated metaphase II oocytes with cytoplasmic aspiration in group I and without cytoplasmic aspiration in group II before sperm injection.
Fertilization rate, oocyte damage rate, and embryo quality.
Normal fertilization rate per injected oocyte was 61.4% in group I compared with 62.5% in group II. The damage rate per injected oocyte was 16.8% in group I compared with 4.6% in group II. Grade I embryos were 24.5% in group I compared with 48.5% in group II.
Cytoplasmic aspiration before sperm injection in ICSI is not essential for oocyte activation. It did not improve the rate of normal fertilization. On the other hand, it increased the damaged oocyte rate and the rate of cytoplasmic fragments.
比较卵胞浆内单精子注射（ICSI）前进行卵胞浆抽吸与不进行抽吸对卵母细胞损伤率、受精率和胚胎质量的影响。
一项关于同胞卵母细胞的随机前瞻性研究。
埃及开罗的埃及体外受精 - 胚胎移植中心。
58例因男性因素不孕且接受了60个ICSI周期的患者。
在精子注射前，对随机分配的中期II卵母细胞，I组进行卵胞浆抽吸后进行卵胞浆内单精子注射，II组不进行卵胞浆抽吸。
受精率、卵母细胞损伤率和胚胎质量。
I组每个注射卵母细胞的正常受精率为61.4%，II组为62.5%。I组每个注射卵母细胞的损伤率为16.8%，II组为4.6%。I组I级胚胎为24.5%，II组为48.5%。
ICSI中精子注射前进行卵胞浆抽吸对卵母细胞激活并非必要。它并未提高正常受精率。另一方面，它增加了受损卵母细胞率和胞质碎片率。
