婴儿肥厚性幽门狭窄中Cajal间质细胞的研究
Study of the interstitial cells of Cajal in infantile hypertrophic pyloric stenosis.
作者信息
Vanderwinden J M, Liu H, De Laet M H, Vanderhaeghen J J
机构信息
Laboratoire de Neurophysiologie et Physiopathologie du Système Nerveux, Faculté de Médecine, Université Libre de Bruxelles.
出版信息
Gastroenterology. 1996 Aug;111(2):279-88. doi: 10.1053/gast.1996.v111.pm8690192.
BACKGROUND & AIMS: The interstitial cells of Cajal form a network in close association with the smooth muscle of the gut. They are regarded as pacemaker cells and might be involved in motility disorders. Their distribution was studied in a common disorder with a dysfunction of the pyloric sphincter called infantile hypertrophic pyloric stenosis.
METHODS
Specimens from 27 infants with pyloric stenosis and 12 controls were processed for immunohistochemistry using a specific antiserum raised against c-kit, a tyrosine kinase receptor expressed by interstitial cells.
RESULTS
In the normal pylorus, numerous interstitial cells were labeled throughout the tissue. In pyloric stenosis, c-kit immunoreactivity was absent in the major part of the tissue. Interstitial cells were observed only in the inner part of the musculature, near the submucosal edge, and in the antrum, at the proximal end of the biopsy specimens.
CONCLUSIONS
The lack of interstitial cells in the pylorus possibly contributes to the motility disturbance of infantile pyloric stenosis.
背景与目的
Cajal间质细胞在肠道平滑肌周围形成一个紧密相连的网络。它们被视为起搏细胞，可能与运动障碍有关。在一种常见的伴有幽门括约肌功能障碍的疾病——婴儿肥厚性幽门狭窄中，对其分布情况进行了研究。
方法
采用针对c-kit（一种由间质细胞表达的酪氨酸激酶受体）产生的特异性抗血清，对27例幽门狭窄婴儿和12例对照的标本进行免疫组织化学处理。
结果
在正常幽门中，整个组织内有大量间质细胞被标记。在幽门狭窄中，大部分组织中缺乏c-kit免疫反应性。仅在肌层内部、靠近黏膜下层边缘以及活检标本近端的胃窦处观察到间质细胞。
结论
幽门中间质细胞的缺失可能导致婴儿幽门狭窄的运动障碍。
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