Chen F, Zhou J, Ritter J K, Bondy C A, Owens I S
Human Genetics Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
Gastroenterology. 1996 Aug;111(2):472-80. doi: 10.1053/gast.1996.v111.pm8690214.
BACKGROUND & AIMS: Heterogeneity in uridine 5'-diphosphate (UDP) glucuronosyltransferase expression across the human hepatic acinus may be important in the manifestation of certain zone-specific chemical hepatotoxicities. Previous immunohistochemical studies suggested that a phenol transferase induced by polycyclic aromatic hydrocarbons may be differentially expressed in centrilobular hepatocytes of rats. The aim of this study was to assess the distribution of the phenol and bilirubin transferases in human liver at the RNA level.
In situ RNA hybridization was used with two human liver samples and specific probes for the phenol transferase RNA, HLUG P1, and the bilirubin transferase RNAs, HUG-Br1 and HUG-Br2.
The highest density signals were observed for the bilirubin transferase RNAs, both appearing to be evenly expressed in hepatocytes across the liver lobule. Slightly higher density of HUG-Br1 message was observed in some centrilobular hepatocytes surrounding larger central vein structures. HLUG P1 RNA was expressed at low levels (approximately fivefold greater than background signal) and was evenly distributed.
The data suggest that a species difference exists in the distribution of the human and rat phenol transferase. No evidence was found for significant zonation in the pattern of expression of either the phenol or bilirubin transferase genes in human liver.
尿苷5'-二磷酸（UDP）葡糖醛酸基转移酶在人肝腺泡中的表达异质性可能在某些区域特异性化学性肝毒性的表现中起重要作用。先前的免疫组织化学研究表明，一种由多环芳烃诱导的酚转移酶在大鼠小叶中央肝细胞中可能有差异表达。本研究的目的是在RNA水平评估酚转移酶和胆红素转移酶在人肝脏中的分布。
对两份人肝脏样本使用原位RNA杂交，以及针对酚转移酶RNA（HLUG P1）和胆红素转移酶RNA（HUG-Br1和HUG-Br2）的特异性探针。
观察到胆红素转移酶RNA的信号密度最高，两者在整个肝小叶的肝细胞中似乎均有均匀表达。在围绕较大中央静脉结构的一些小叶中央肝细胞中观察到HUG-Br1信息的密度略高。HLUG P1 RNA表达水平较低（比背景信号高约5倍）且分布均匀。
数据表明人和大鼠酚转移酶的分布存在种属差异。未发现人肝脏中酚转移酶或胆红素转移酶基因表达模式有明显的区域化证据。
