Kogure K, Zhang Y Q, Kanzaki M, Omata W, Mine T, Kojima I
First Department of Surgery, Gunma University School of Medicine, Gunma University, Maebashi, Japan.
Hepatology. 1996 Aug;24(2):361-6. doi: 10.1002/hep.510240212.
When 1 microgram 125I-follistatin was administered into a rat intravenously, radioactivity levels in serum decreased rapidly. Analysis with a biexponential equation showed that the initial half-life and the terminal half-life were 4.0 and 130.8 minutes, respectively. After 2 hours of infusion, approximately 9% of the follistatin infused remained in the liver, which was much more than that in kidney, spleen, pancreas, intestine, or lung. Autoradiography of the liver obtained at 24 hours of infusion revealed that numerous grains were located in parenchymal cells. Radioactivity of 125I-follistatin in the liver remained elevated until 72 hours and declined markedly thereafter. When a booster shot of 125I-follistatin was administered at 72 hours, radioactivity in the liver at 120 hours was markedly increased compared with that in rats that received a single shot of 125I-follistatin. We then examined the effect of intravenous infusion of follistatin on liver regeneration after hepatectomy of 70%. Immediately after the hepatectomy, either 1 microgram follistatin or saline was infused intravenously. In some rats, a booster shot was infused at 72 hours. After 120 hours of hepatectomy of 70%, remnant liver weight, liver regeneration rate, and DNA content were significantly (P < .05) higher in rats that received a booster shot of follistatin at 72 hours than those in control rats. These results indicate that follistatin administered intravenously accumulates in the liver and promotes liver regeneration after partial hepatectomy.
将1微克¹²⁵I - 卵泡抑素静脉注射到大鼠体内后，血清中的放射性水平迅速下降。用双指数方程分析表明，初始半衰期和终末半衰期分别为4.0分钟和130.8分钟。输注2小时后，输注的卵泡抑素中约9%留在肝脏中，这比肾脏、脾脏、胰腺、肠道或肺中的含量要高得多。输注24小时后获得的肝脏放射自显影片显示，实质细胞中有大量颗粒。¹²⁵I - 卵泡抑素在肝脏中的放射性一直升高到72小时，此后明显下降。当在72小时给予¹²⁵I - 卵泡抑素加强注射时，与接受单次¹²⁵I - 卵泡抑素注射的大鼠相比，120小时时肝脏中的放射性明显增加。然后，我们研究了静脉输注卵泡抑素对70%肝切除术后肝脏再生的影响。肝切除术后立即静脉输注1微克卵泡抑素或生理盐水。在一些大鼠中，在72小时给予加强注射。在70%肝切除术后120小时，72小时接受卵泡抑素加强注射的大鼠的残余肝脏重量、肝脏再生率和DNA含量显著（P <.05）高于对照大鼠。这些结果表明，静脉注射的卵泡抑素在肝脏中蓄积并促进部分肝切除术后的肝脏再生。
