Hale C, Bartholomew M, Taylor V, Stables J, Topley P, Tite J
Molecular Immunology, Wellcome Research Laboratories, Kent, UK.
Immunology. 1996 Jun;88(2):183-90. doi: 10.1111/j.1365-2567.1996.tb00003.x.
Cloning of the CD52 from a B-lymphocyte tumour cDNA library revealed two closely related sequences differing only at two amino acids C-terminal to the proposed point of glycosylphosphatidylinositol (GPI)-linkage. When transfected into CHO cells only one of these sequences gave high-level expression of the antigen recognized by the prototypic anti-CD52 antibody CAMPATH-1 whereas in JURKAT cells good expression levels were obtained with both sequences. Fusion of the sequence from the second sequence to DNA encoding the extracellular domain of CD4 indicated that this sequence was capable of directing GPI linkage. The possible implications for the function of CD52 and serotherapy with anti-CD52 antibodies are discussed.
从B淋巴细胞肿瘤cDNA文库中克隆CD52，发现了两个紧密相关的序列，它们仅在糖基磷脂酰肌醇（GPI）连接位点C端的两个氨基酸处有所不同。当转染到CHO细胞中时，这些序列中只有一个能使原型抗CD52抗体CAMPATH-1识别的抗原高水平表达，而在JURKAT细胞中，两个序列都能获得良好的表达水平。将第二个序列的序列与编码CD4细胞外结构域的DNA融合，表明该序列能够指导GPI连接。文中讨论了CD52功能以及抗CD52抗体血清疗法的可能意义。
