Jayakumar R, Chandramohan A, Govindrajan R, Padmanaban V D
Madras Veterinary College.
Indian J Public Health. 1995 Jan-Mar;39(1):8-11.
A rapid dot enzyme immuno assay (Dot ELISA) for rabies antibody estimation has been standardized in which nitrocellulose sheet has been used as a solid support absorbing a commercial tissue culture rabies vaccine as antigen. The test was compared with a standard plate ELISA test. The results were comparable and the student "t' test for proportion revealed that plate ELISA test is significantly better (P 0.05) when compared to Dot ELISA for the number of sera with titre < 0.5 Iu/ml and in the case of > 0.5 IU/ml Dot ELISA is found to be better over plate ELISA test at the same significance level.
一种用于狂犬病抗体检测的快速斑点酶免疫测定法（Dot ELISA）已标准化，其中硝酸纤维素膜被用作固相载体，吸附一种市售组织培养狂犬病疫苗作为抗原。该检测方法与标准平板ELISA检测进行了比较。结果具有可比性，比例的学生t检验显示，对于滴度<0.5国际单位/毫升的血清数量，平板ELISA检测明显更好（P<0.05）；而在滴度>0.5国际单位/毫升的情况下，在相同显著性水平下，Dot ELISA比平板ELISA检测更好。
