Takeda M, Okushiba T, Satoh T, Kuniyoshi S, Morishita C, Ichimura Y
Department of Applied Microbial Technology, Kumamoto Institute of Technology.
J Biochem. 1995 Sep;118(3):607-13. doi: 10.1093/oxfordjournals.jbchem.a124953.
Chromosome fragmentation, ATP1 disruption, and Southern blot analyses of total DNAs and prime clones of chromosome II showed that three identical ATP1s are present, directing from the telomere to the centromere on the 35-55 kb far from the left telomere sequence of chromosome II. That is, the coding and 5'-, 3'-non-coding regions of ATP1 are repeated 3 times at approximately 7 kb intervals. These three ATP1s are expressed, and one and two ATP1s-disrupted strains, respectively, showed ca.70 and 40% decreases in their ATPase activities and alpha subunit contents, compared to those of the wild type, DC-5 or W303-1A strain, but could grow on glycerol.
染色体片段化、ATP1破坏以及对总DNA和二号染色体原始克隆的Southern印迹分析表明，存在三个相同的ATP1，它们从端粒指向着丝粒，位于距离二号染色体左端粒序列35 - 55 kb处。也就是说，ATP1的编码区以及5' -、3' -非编码区以大约7 kb的间隔重复了3次。这三个ATP1均有表达，与野生型DC - 5或W303 - 1A菌株相比，分别破坏了一个和两个ATP1的菌株，其ATP酶活性和α亚基含量分别下降了约70%和40%，但仍能在甘油上生长。
