用于检测人类隐源性肝炎中GB病毒C的一致性寡核苷酸引物。

Consensus oligonucleotide primers for the detection of GB virus C in human cryptogenic hepatitis.

作者信息

Leary T P, Muerhoff A S, Simons J N, Pilot-Matias T J, Erker J C, Chalmers M L, Schlauder G G, Dawson G J, Desai S M, Mushahwar I K

机构信息

Virus Discovery Group, Abbott Laboratories, North Chicago, IL 60064, USA.

出版信息

J Virol Methods. 1996 Jan;56(1):119-21. doi: 10.1016/0166-0934(95)01956-1.

DOI:10.1016/0166-0934(95)01956-1

PMID:8690760

Abstract

Recently, sequences from a putative member of the Flaviviridae, GB virus C (GBV-C), were isolated from the serum of patients with cryptogenic hepatitis. These sequences were 83-99% identical at the nucleotide level. Because of the divergence between these GBV-C isolates, it is likely that the PCR-based detection assay yields false negatives, underestimating dramatically the true prevalence of GBV-C in human hepatitis. We report the design of a GBV-C consensus oligonucleotide primer pair that is superior to those originally described. These primers identify GBV-C sequences in cases of cryptogenic hepatitis, allowing a better estimation of the prevalence of this virus in human populations.

摘要

最近，从隐源性肝炎患者血清中分离出黄病毒科假定成员GB病毒C（GBV-C）的序列。这些序列在核苷酸水平上有83%-99%的同一性。由于这些GBV-C分离株之间存在差异，基于PCR的检测方法很可能产生假阴性结果，从而极大地低估了GBV-C在人类肝炎中的真实流行率。我们报告了一种GBV-C一致性寡核苷酸引物对的设计，该引物对比最初描述的引物对更优。这些引物可识别隐源性肝炎病例中的GBV-C序列，从而能更好地估计该病毒在人群中的流行率。