Vendeira P, Pignatelli D, Neves D, Magalhães M M, Magalhães M C, Ho M M, Vinson G P
Department of Histology and Embryology, University of Porto, Portugal.
J Endocrinol. 1996 Jun;149(3):497-502. doi: 10.1677/joe.0.1490497.
Adrenal gland autotransplantation, an interesting model of adrenal regeneration, provides the reconstruction of distinct functional and morphological zonae. An immunohistochemical study of the adrenal gland of adult male rats after autotransplantation and endothelin-1 (ET-1) stimulation was carried out. The technique involved total adrenalectomy and immediate autotransplantation of small adrenal pieces under the skin of the dorsal region. The animals were killed 90 days after the autotransplantation and 1 h after intravenous ET-1 administration. Sections of recovered adrenal grafts were incubated with IZAb, a monoclonal antibody which interacts with an antigen (IZAg) predominantly found in rat adrenal inner zones. Saline-treated control autotransplanted animals showed IZAb immunostaining in almost all adrenocortical tissue, with the exception of small clusters of cells beneath the capsule. ET-1-treated animals exhibited an extended zone devoid of immunostaining and located in the subcapsular area. In addition, ET-1-stimulated animals showed significant increases in aldosterone as well as corticosterone concentrations in plasma. These results revealed that ET-1 stimulated the development of an extended subcapsular zone lacking IZAg expression, an effect that suggests its role in zona glomerulosa induction in these animals.
肾上腺自体移植是一种有趣的肾上腺再生模型，可重建不同的功能和形态区域。对成年雄性大鼠自体移植并经内皮素-1（ET-1）刺激后的肾上腺进行了免疫组织化学研究。该技术包括全肾上腺切除术，并将小的肾上腺组织立即自体移植到背部皮下。在自体移植90天后和静脉注射ET-1 1小时后处死动物。将回收的肾上腺移植物切片与IZAb一起孵育，IZAb是一种单克隆抗体，可与主要在大鼠肾上腺内部区域发现的一种抗原（IZAg）相互作用。用生理盐水处理的对照自体移植动物在几乎所有肾上腺皮质组织中均显示IZAb免疫染色，但在被膜下有小细胞簇除外。经ET-1处理的动物在被膜下区域出现一个无免疫染色的扩展区。此外，经ET-1刺激的动物血浆中醛固酮和皮质酮浓度显著升高。这些结果表明，ET-1刺激了一个缺乏IZAg表达的扩展被膜下区的发育，这一效应表明其在这些动物球状带诱导中的作用。
