Ankri S, Reyes O, Leblon G
Institut de Génétique et Microbiologie, Université de Paris-Sud, Orsay, France.
Plasmid. 1996 Jan;35(1):62-6. doi: 10.1006/plas.1996.0007.
Highly DNA-restrictive Corynebacteria can be transformed with DNA made in vitro by PCR amplification of a sequence that contains the replication origin of pBL1, a plasmid common to many Corynebacteria. In all strains examined, the transformation efficiencies of PCR-synthetized DNA equal or improve the performances of heterologous DNA extracted from wild-type and dam(-)-dcm-strains of Escherichia coli. The transformation efficiencies obtained with PCR-made DNA may be high enough to permit its general application to experiments of gene integration.
高度限制DNA的棒状杆菌可以用通过PCR扩增包含pBL1复制起点的序列体外合成的DNA进行转化，pBL1是许多棒状杆菌共有的质粒。在所有检测的菌株中，PCR合成DNA的转化效率等同于或优于从大肠杆菌野生型和dam(-)-dcm-菌株中提取的异源DNA的表现。用PCR制备的DNA获得的转化效率可能足够高，以允许其普遍应用于基因整合实验。
