Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Pühler A
Department of Genetics, University of Bielefeld, Germany.
FEMS Microbiol Lett. 1994 Nov 1;123(3):343-7. doi: 10.1111/j.1574-6968.1994.tb07246.x.
Efficient electroporation of Escherichia coli with plasmid DNA isolated from Corynebacterium glutamicum depends on the use of Mcr-deficient E. coli strains. The transformation frequency increased nearly 800-fold when the Mcr-deficient E. coli DH5 alpha MCR was used instead of E. coli DH5 alpha. We used E. coli strains with different mutations in the methyl-specific restriction systems to show that McrBC-deficiency is sufficient to generate this effect. The results imply that C. glutamicum DNA contains methylcytosine in specific sequences recognized by the E. coli McrBC system.
用从谷氨酸棒杆菌中分离的质粒DNA对大肠杆菌进行高效电穿孔取决于使用Mcr缺陷型大肠杆菌菌株。当使用Mcr缺陷型大肠杆菌DH5α MCR而非大肠杆菌DH5α时，转化频率增加了近800倍。我们使用在甲基特异性限制系统中具有不同突变的大肠杆菌菌株来表明McrBC缺陷足以产生这种效应。结果表明，谷氨酸棒杆菌DNA在大肠杆菌McrBC系统识别的特定序列中含有甲基胞嘧啶。
