Tsai T H, Chou C J, Tsai T R, Chen C F
Department of Pharmacology, National Research Institute of Chinese Medicine, Shih-Pai, Taipei, Taiwan.
Planta Med. 1996 Jun;62(3):263-6. doi: 10.1055/s-2006-957874.
A high-performance liquid chromatographic method has been developed for the determination of wogonin in the rat plasma. After addition of an internal standard (clomipramine), plasma was deproteinized by acetonitrile and centrifuged for sample clean-up. The supernatant was separated by a reversed-phase column chromatography, identified by a photodiode-array detector, and quantified by ultraviolet detection at a wavelength of 275 nm. Acetonitrile-water-diethylamine (50:50:0.1, v/v/v, pH 3.0 adjusted by orthophosphoric acid) was used as the mobile phase. The method was applied to the pharmacokinetic study of wogonin in rats after a dose of mg/kg by intravenous administration. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration time profile.
已开发出一种高效液相色谱法用于测定大鼠血浆中的汉黄芩素。加入内标（氯米帕明）后，用乙腈使血浆脱蛋白并离心以净化样品。上清液通过反相柱色谱分离，用光电二极管阵列检测器进行鉴定，并通过在275nm波长处的紫外检测进行定量。乙腈 - 水 - 二乙胺（50:50:0.1，v/v/v，用正磷酸调节pH至3.0）用作流动相。该方法应用于大鼠静脉注射mg/kg剂量汉黄芩素后的药代动力学研究。从血浆浓度 - 时间曲线观察到一种双相现象，即快速分布后接着是较慢的消除相。
