Peng H W, Cheng F C, Huang Y T, Chen C F, Tsai T H
Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
J Chromatogr B Biomed Sci Appl. 1998 Sep 4;714(2):369-74. doi: 10.1016/s0378-4347(98)00204-7.
An isocratic high-performance liquid chromatographic method with ultraviolet detection was utilized for the investigation of the pharmacokinetics of naringenin and its glucuronide conjugate in rat plasma and brain tissue. Plasma and brain tissue were deproteinized by acetonitrile, then centrifuged for sample clean-up. The drugs were separated by a reversed-phase C18 column with a mobile phase consisting of acetonitrile-orthophosphoric acid solution (pH 2.5-2.8) (36:64, v/v). The detection limits of naringenin in rat plasma and brain tissue were 50 ng/ml and 0.4 microg/g, respectively. The glucuronide conjugate of naringenin was evaluated by the deconjugated enzyme beta-glucuronidase. The naringenin conjugation ratios in rat plasma and brain tissue were 0.86 and 0.22, respectively, 10 min after naringenin (20 mg/kg, i.v.) administration. The mean naringenin conjugation ratio in plasma was approximately four fold that in brain tissue.
采用具有紫外检测的等度高效液相色谱法研究柚皮素及其葡糖醛酸缀合物在大鼠血浆和脑组织中的药代动力学。血浆和脑组织用乙腈进行脱蛋白处理，然后离心以净化样品。药物通过反相C18柱进行分离，流动相由乙腈 - 正磷酸溶液（pH 2.5 - 2.8）（36:64，v/v）组成。柚皮素在大鼠血浆和脑组织中的检测限分别为50 ng/ml和0.4 μg/g。柚皮素的葡糖醛酸缀合物通过去共轭酶β-葡糖醛酸酶进行评估。在静脉注射（20 mg/kg）柚皮素后10分钟，大鼠血浆和脑组织中柚皮素的缀合率分别为0.86和0.22。血浆中柚皮素的平均缀合率约为脑组织中的四倍。
