Taus V
Dept. of Histology and Embryology, Medical Faculty, Charles University, Hradec Králové.
Sb Ved Pr Lek Fak Karlovy Univerzity Hradci Kralove. 1995;38(4):145-65.
Unique character of brain tumors and problems inherent in different concepts of histological classification are impediments to effective histoprognosis. Large place for subjectivity may be diminished by integration of kinetics data into evaluation of tumor samples. Methods of measuring cell proliferation include immunohistochemical detection of halogenated or tritiated pyrimidine analogues after intravenous injection and nuclear antigens associated with the cell cycle, other methods are selective silver staining and modified flow cytometry. In this study, the literature concerning the use of proliferation markers in neurooncology is reviewed and the problems consisting in used methods are pointed out. The most reliable markers and techniques are recommended and situations of special importance for the cell proliferation markers use specified.
脑肿瘤的独特特征以及不同组织学分类概念中固有的问题是有效进行组织学预后判断的障碍。将动力学数据纳入肿瘤样本评估中，可以减少主观性的较大影响。测量细胞增殖的方法包括静脉注射后对卤化或氚化嘧啶类似物以及与细胞周期相关的核抗原进行免疫组化检测，其他方法有选择性银染色和改良流式细胞术。在本研究中，回顾了神经肿瘤学中使用增殖标志物的相关文献，并指出了所用方法存在的问题。推荐了最可靠的标志物和技术，并明确了细胞增殖标志物使用中特别重要的情况。
