Funyu T, Kimura T, Yamamoto T, Ota K, Shoji M, Ohta M, Sato K, Abe K
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Tohoku J Exp Med. 1995 Sep;177(1):73-87. doi: 10.1620/tjem.177.73.
To assess whether atrial natriuretic peptide (ANP) plays a role in the natriuresis induced by interleukin 1 beta (IL-1 beta), the following experiments were carried out. Experiment (Ex) I: IL-1 beta (7.5 micrograms/kg BW) was given intravenously (i.v.) in conscious hydrated rats (n = 6). Plasma ANP, vasopressin (AVP) osmolality (Posm), Na and K, urine Na (UNa V) and K excretion (UK V), osmolality and flow (UF), and mean arterial blood pressure (MABP) and heart rate were simultaneously determined. In the control group (n = 6), the drug was omitted, and the same protocols were carried out. Ex II: Three mg/kg BW of the specific ANP antagonist, HS-142-1 (HS), was administered i.v. and then, IL-1 beta (7.5 micrograms/kg BW) was given i.v. (n = 6). In the HS alone group (n = 6), IL-1 beta was omitted. The experimental protocols were the same as those in Ex I. IL-1 beta increased significantly plasma ANP and AVP and UNa V, but not UF, accompanied by decreases in Posm and UKV and increases in MABP (ExI). HS inhibited the natriuresis mediated by IL-1 beta, despite increases in plasma ANP and had no influence on plasma AVP and MABP. In the control (ExI) and HS alone (ExII) groups, these parameters did not change, except for decreases in Posm in both groups and increased plasma ANP in the latter. These results suggest that plasma ANP may play an essential role in the IL-1 beta-mediated natriuresis.
为评估心房利钠肽（ANP）是否在白细胞介素1β（IL-1β）诱导的利钠作用中发挥作用，进行了以下实验。实验一：在清醒的水合大鼠（n = 6）中静脉注射（i.v.）IL-1β（7.5微克/千克体重）。同时测定血浆ANP、血管加压素（AVP）、渗透压（Posm）、钠和钾、尿钠（UNa V）和钾排泄（UK V）、渗透压和流量（UF）以及平均动脉血压（MABP）和心率。在对照组（n = 6）中，省略药物，执行相同的方案。实验二：静脉注射3毫克/千克体重的特异性ANP拮抗剂HS-142-1（HS），然后静脉注射IL-1β（7.5微克/千克体重）（n = 6）。在仅给予HS组（n = 6）中，省略IL-1β。实验方案与实验一相同。IL-1β显著增加血浆ANP、AVP和UNa V，但不增加UF，同时伴有Posm和UKV降低以及MABP升高（实验一）。HS抑制IL-1β介导的利钠作用，尽管血浆ANP增加，且对血浆AVP和MABP无影响。在对照组（实验一）和仅给予HS组（实验二）中，除两组的Posm降低和后者的血浆ANP升高外，这些参数均未改变。这些结果表明，血浆ANP可能在IL-1β介导的利钠作用中起重要作用。
