Krupicer I, Velebný S, Legáth J
Parasitological Institute, Slovak Academy of Sciences, Kosice, Slovak Republic.
Vet Med (Praha). 1996 Apr;41(4):103-6.
A group of 14 improved Valashka lambs, aged 4 months, body weight 14-16 kg, were used in the experiment studying the effect of subchronic heavy metal intoxication on the course of experimental fasciolosis (Fasciola hepatica). The animals were divided into two groups, each of 7 lambs, 3 lambs--ewes and 4 lambs--rams. Each animal in group I was given orally for 27 days gelatinous capsules containing heavy metal emissions of the following composition: 4.5 mg Hg, 2.9 mg Pb, 147.8 mg Cu, 9.8 mg Zn, 0.9 mg Cr and 0.04 mg Cd per animal and day. Group II served as control. On Day 27, when the administration of heavy metals ceased, each animal in both groups received 250 F. hepatica metacercariae. The animals were sacrificed on Day 110 post infection, their liver necropsied and parenchymatous organs and muscles taken to examine the content of heavy metals. The heavy metal concentrations are presented in Tab. I. In the emission-intoxicated group, the maximum permissible reference level of mercury in the kidneys, liver and muscles was exceeded on the average by 1.94, 0.87 and 0.020 mg Hg/kg, respectively. Cu exceeded the reference level only in the liver, with 261.3 mg Cu/kg. In the kidneys and muscles, Cu was below the reference levels. An analogous situation was with Zn, Cr, and Cd. The F. hepatica infection mean intensity in this animal group was 38.3 +/- 3.01 specimens (min. 34 - max. 46 specimens). In control group, the heavy metal levels were several times lower than the reference values and the infection mean intensity was 27 +/- 5.4 (min. 19 - max. 35 specimens). In subchronically intoxicated animals, the reference values for mercury were exceeded and so were partially those of copper in the parenchymatous organs and muscles. The F. hepatica infection mean intensity also increased, compared with control. The results have proved the negative effect of the metal emissions on the animal immune system, which was manifested by the increased infection intensity.
选用14只4月龄、体重14 - 16千克的改良瓦拉什卡羔羊，用于研究亚慢性重金属中毒对实验性肝片吸虫病（肝片吸虫）病程的影响。将动物分为两组，每组7只羔羊，其中3只为母羊，4只为公羊。第一组的每只动物连续27天口服含有以下成分重金属排放物的凝胶胶囊：每只动物每天4.5毫克汞、2.9毫克铅、147.8毫克铜、9.8毫克锌、0.9毫克铬和0.04毫克镉。第二组作为对照。在第27天，重金属给药停止时，两组的每只动物均感染250只肝片吸虫囊蚴。在感染后第110天处死动物，对其肝脏进行尸检，并取实质器官和肌肉检测重金属含量。重金属浓度见表一。在排放物中毒组，肾脏、肝脏和肌肉中汞的最大允许参考水平平均分别超出1.94、0.87和0.020毫克汞/千克。铜仅在肝脏中超过参考水平，为261.3毫克铜/千克。在肾脏和肌肉中，铜低于参考水平。锌、铬和镉的情况类似。该动物组肝片吸虫感染平均强度为38.3±3.01个虫体（最小值34 - 最大值46个虫体）。对照组中，重金属水平比参考值低几倍，感染平均强度为27±5.4（最小值19 - 最大值35个虫体）。在亚慢性中毒动物中，实质器官和肌肉中汞超过参考值，铜也部分超过参考值。与对照组相比，肝片吸虫感染平均强度也增加了。结果证明了金属排放物对动物免疫系统有负面影响，表现为感染强度增加。
