Pierard D, De Meyer A, Rosseel P, Van Cauwenbergh M, Struelens M J, Delmee M, Goossens H, Claeys G, Glupczynski Y, Verbist L, Melin P, Lauwers S
Department of Microbiology, Academisch Ziekenhuis Vrije Universteit, Brussel.
Acta Clin Belg. 1996;51(2):70-9. doi: 10.1080/17843286.1996.11718489.
The activity of amoxycillin/clavulanate (Augmentin) and ticarcillin/ clavulanate (Timentin) was tested against 351 strict anaerobic clinical isolates collected from September 1993 to April 1994 in eight Belgian university hospitals and compared with that of 8 other antibiotics using the NCCLS reference agar dilution procedure. Production of beta-lactamase was detected by the nitrocefin test in 48% of the isolates. At NCCLS-recommended breakpoints, more than 90% of isolates were susceptible to amoxycillin/clavulanate, ticarcillin/clavulanate, piperacillin/tazobactam, imipenem, chloramphenicol and metronidazole but only 77%, 72% and 48% to cefoxitin, clindamycin and penicillin, respectively. In comparison with the results of a similar survey conducted in 1987 no major changes in susceptibility were observed except for the susceptibility to clindamycin that declined from 83% to 72% overall, and from 83% to 66% in the B. fragilis group. Furthermore one isolate of Clostridium clostridioforme was found produce beta-lactamase and few B. fragilis group isolates showed reduced susceptibility to metronidazole.
1993年9月至1994年4月期间，从比利时八所大学医院收集了351株严格厌氧临床分离株，采用美国国家临床实验室标准化委员会（NCCLS）参考琼脂稀释法，检测了阿莫西林/克拉维酸（奥格门汀）和替卡西林/克拉维酸（特泯菌）对这些菌株的活性，并与其他8种抗生素进行了比较。通过硝基头孢菌素试验检测到48%的分离株产生β-内酰胺酶。按照NCCLS推荐的断点，超过90%的分离株对阿莫西林/克拉维酸、替卡西林/克拉维酸、哌拉西林/他唑巴坦、亚胺培南、氯霉素和甲硝唑敏感，但对头孢西丁、克林霉素和青霉素的敏感率分别仅为77%、72%和48%。与1987年进行的类似调查结果相比，除了对克林霉素的敏感性总体从83%降至72%，在脆弱拟杆菌组中从83%降至66%外，未观察到敏感性有重大变化。此外，发现一株艰难梭状芽胞杆菌分离株产生β-内酰胺酶，少数脆弱拟杆菌组分离株对甲硝唑的敏感性降低。
