Ong S, Pidgeon C
Department of Medicinal Chemistry, School of Pharmacy, Purdue University, West Lafayette, Indiana 47907, USA.
Anal Chem. 1995 Jul 1;67(13):2119-28. doi: 10.1021/ac00109a034.
The solute retention mechanism on immobilized artificial membranes (IAMs) was studied using three different IAM.PC phases. IAMs were prepared by immobilizing either single-chain or double-chain phosphatidylcholine (PC) ligands. Solute affinity for the single-chain IAM.PC columns (with a ligand density of 127 mumol of PC/g of IAM) was 3-fold lower compared to solute affinity on the double-chain IAM.PC column (with a ligand density of 98 mumol of PC/g of IAM). This suggests that the solute retention on IAMs is dominated by a solute partitioning mechanism. Temperature-dependent studies indicated that the thermodynamics of solute partitioning is similar on both the single-chain and double-chain IAM.PC surfaces. For a set of phenol derivatives, the partitioning into IAM.PC surfaces is both enthalpy and entropy driven. For beta-blockers, the partitioning into IAM.PC surfaces is entropy driven. The free energy of solute partitioning into IAMs correlates very well with the free energy of solute partitioning into liposomes.
使用三种不同的固定化人工膜（IAM）.PC相研究了溶质在IAM上的保留机制。通过固定单链或双链磷脂酰胆碱（PC）配体制备IAM。与双链IAM.PC柱（配体密度为98 μmol PC/g IAM）相比，溶质对单链IAM.PC柱（配体密度为127 μmol PC/g IAM）的亲和力低3倍。这表明溶质在IAM上的保留主要由溶质分配机制主导。温度依赖性研究表明，溶质在单链和双链IAM.PC表面上的分配热力学相似。对于一组酚类衍生物，进入IAM.PC表面的分配是由焓和熵驱动的。对于β受体阻滞剂，进入IAM.PC表面的分配是由熵驱动的。溶质分配到IAM中的自由能与溶质分配到脂质体中的自由能非常好地相关。
