Takahashi H, Kirsch J R, Okada T, Traystman R J
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.
Anesth Analg. 1996 Aug;83(2):359-65. doi: 10.1097/00000539-199608000-00027.
Cerebrovascular responses to physiologic and pharmacologic stimuli vary between laboratories using different strains of the same species. We tested whether the cerebral blood flow (CBF) response to 1% halothane or hypercapnia is strain-dependent in rats. Age-matched adult male (n = 14 of each strain) Wistar, Wistar-Kyoto (WKY), and spontaneously hypertensive rats (SHR) were anesthetized with pentobarbital and mechanically ventilated. Under baseline conditions blood flow to cerebrum (microspheres) in WKY (66 +/- 5 mL.min-1.100 g-1) was less than (P < 0.05) in Wistar (88 +/- 5 mL.min-1.100g-1) and SHR (83 +/- 5 mL.min-1.100 g-1). Blood flow to brainstem was greater (P < 0.05) in Wistar (106 +/- 8 mL. min-1.100 g-1) than in WKY (71 +/- 5 mL.min-1. 100 g-1) and SHR (84 +/- 4 mL.min-1.100 g-1). In the halothane protocol (n = 8 each strain), administration of 1% halothane, during normocapnia, increased blood flow to the cerebrum in WKY (64 +/- 6 to 120 +/- 12 mL.min-1.100 g-1, P < 0.05) and SHR (78 +/- 6 to 115 +/- 8 mL.min-1.100 g-1, P < 0.05) but not Wistar rats (88 +/- 8 to 102 +/- 5 mL.min-1.100 g-1, not significant). Discontinuing halothane caused blood flow to return to baseline values. In the hypercapnia protocol (n = 6 each strain), exposure to 3% CO2 (to achieve a PaCO2 of 50-55 mm Hg) and 6% CO2 (to achieve a PaCO2 of 60-70 mm Hg) caused blood flow to the cerebrum to increase in Wistar (87 +/- 11 to 112 +/- 15 to 162 +/- 23) to similar amount as observed in WKY (69 +/- 7 to 115 +/- 13 to 162 +/- 23 mL.min-1. 100 g-1) but less than that observed in SHR (89 +/- 7 to 174 +/- 24 to 237 +/- 28 mL.min-1.100 g-1). These data demonstrate that the cerebral hyperemic response to vasodilator stimuli is strain-dependent in rats.
在使用同一物种不同品系的不同实验室中，脑血管对生理和药理刺激的反应存在差异。我们测试了大鼠对1%氟烷或高碳酸血症的脑血流量（CBF）反应是否依赖于品系。将年龄匹配的成年雄性（每个品系n = 14）Wistar大鼠、Wistar - Kyoto（WKY）大鼠和自发性高血压大鼠（SHR）用戊巴比妥麻醉并进行机械通气。在基线条件下，WKY大鼠大脑血流量（微球法）为（66±5 mL·min⁻¹·100 g⁻¹），低于（P < 0.05）Wistar大鼠（88±5 mL·min⁻¹·100 g⁻¹）和SHR大鼠（83±5 mL·min⁻¹·100 g⁻¹）。Wistar大鼠脑干血流量（106±8 mL·min⁻¹·100 g⁻¹）高于（P < 0.05）WKY大鼠（71±5 mL·min⁻¹·100 g⁻¹）和SHR大鼠（84±4 mL·min⁻¹·100 g⁻¹）。在氟烷实验方案中（每个品系n = 8），在正常碳酸血症期间给予1%氟烷，可使WKY大鼠大脑血流量增加（从64±6至120±12 mL·min⁻¹·100 g⁻¹，P < 0.05）以及SHR大鼠（从78±6至115±8 mL·min⁻¹·100 g⁻¹，P < 0.05），但Wistar大鼠大脑血流量（从88±8至102±5 mL·min⁻¹·100 g⁻¹）无显著增加。停止使用氟烷后，血流量恢复到基线值。在高碳酸血症实验方案中（每个品系n = 6），暴露于3% CO₂（使动脉血二氧化碳分压[PaCO₂]达到50 - 55 mmHg）和6% CO₂（使PaCO₂达到60 - 70 mmHg）时，Wistar大鼠大脑血流量增加（从87±11至112±15至162±23），与WKY大鼠（69±7至115±13至162±23 mL·min⁻¹·100 g⁻¹）增加量相似，但低于SHR大鼠（89±7至174±24至237±28 mL·min⁻¹·100 g⁻¹）。这些数据表明，大鼠对血管舒张刺激的脑充血反应依赖于品系。
