Henke J, Willker W, Engelmann J, Leibfritz D
Institut für Organische Chemie, Universität Bremen, Germany.
Anticancer Res. 1996 May-Jun;16(3B):1417-27.
As the cell membranes are one of the targets of drug treatment in cancer cells, their membrane composition and variations in this composition need to be analyzed. Gradient selected 2D-NMR inverse heteronuclear chemical shift correlations are have described, which offer optimum sensitivity combined with a high reliability for unequivocal signal assignment in proton, carbon and phosphorous spectra, concerning neutral lipids and in particular phospholipids. Additionally, an extraction procedure is presented to extract subsequently the water soluble (PCA extract) and lipophilic metabolites (chloroform/methanol extract) from the same cell batch.
由于细胞膜是癌细胞药物治疗的靶点之一，因此需要分析其膜组成及其组成的变化。已描述了梯度选择二维核磁共振反相异核化学位移相关性，它在质子、碳和磷谱中，对于中性脂质尤其是磷脂，提供了最佳灵敏度以及高可靠性以进行明确的信号归属。此外，还介绍了一种提取方法，可从同一批细胞中依次提取水溶性（PCA提取物）和亲脂性代谢物（氯仿/甲醇提取物）。
