Douzi Wafa, Guillot Xavier, Bon Delphine, Seguin François, Boildieu Nadège, Wendling Daniel, Tordi Nicolas, Dupuy Olivier, Dugué Benoit
Laboratoire «Mobilité, Vieillissement, Exercice (MOVE)-EA6314», Faculté des Sciences du Sport, Université de Poitiers, 8 Allée Jean Monnet, 86000 Poitiers, France.
Department of Rheumatology, Felix Guyon University Hospital, 97400 Saint-Denis, France.
Metabolites. 2020 Nov 13;10(11):460. doi: 10.3390/metabo10110460.
Rehabilitation using cryotherapy has widely been used in inflammatory diseases to relieve pain and decrease the disease activity. The aim of this study was to explore the metabolite changes in inflammatory knee-joint synovial fluids following local cryotherapy treatment (ice or cold CO). We used proton nuclear magnetic resonance (H NMR) spectroscopy to assess the metabolite patterns in synovial fluid (SF) in patients with knee arthritis ( = 46) before (D0) and after (D1, 24 h later) two applications of local cryotherapy. Spectra from aqueous samples and organic extracts were obtained with an 11.75 Tesla spectrometer. The metabolite concentrations within the SF were compared between D1 and D0 using multiple comparisons with the application of a false discovery rate (FDR) adjusted at 10% for each metabolite. A total of 32 metabolites/chemical structures were identified including amino acids, organic acids, fatty acids or sugars. Pyruvate, alanine, citrate, threonine was significantly higher at D1 vs D0 ( < 0.05). Tyrosine concentration significantly decreases after cryotherapy application ( < 0.001). We did not observe any effect of gender and cooling technique on metabolite concentrations between D0 and D1 ( > 0.05). The present study provides new insight into a short-term effect of cold stimulus in synovial fluid from patients with knee arthritis. Our observations suggest that the increased level of metabolites involved in energy metabolism may explain the underlying molecular pathways that mediate the antioxidant and anti-inflammatory capacities of cryotherapy.
冷冻疗法康复已广泛应用于炎症性疾病，以缓解疼痛并降低疾病活动度。本研究的目的是探讨局部冷冻疗法（冰敷或冷二氧化碳）治疗后炎性膝关节滑液中的代谢物变化。我们使用质子核磁共振（H NMR）光谱法评估了46例膝关节炎患者在两次局部冷冻疗法治疗前（D0）和治疗后（D1，24小时后）滑液（SF）中的代谢物模式。使用11.75特斯拉光谱仪获得水性样品和有机提取物的光谱。使用多重比较法，对每个代谢物在错误发现率（FDR）调整为10%的情况下比较D1和D0之间SF中的代谢物浓度。共鉴定出32种代谢物/化学结构，包括氨基酸、有机酸、脂肪酸或糖类。与D0相比，丙酮酸、丙氨酸、柠檬酸、苏氨酸在D1时显著升高（P<0.05）。冷冻疗法应用后酪氨酸浓度显著降低（P<0.001）。我们未观察到性别和冷却技术对D0和D1之间代谢物浓度有任何影响（P>0.05）。本研究为膝关节炎患者滑液中冷刺激的短期效应提供了新的见解。我们的观察结果表明，参与能量代谢的代谢物水平升高可能解释了介导冷冻疗法抗氧化和抗炎能力的潜在分子途径。
