Varma Shreya, Gulati Khushi Ash, Sriramakrishnan Janani, Ganla Riyaa Kedar, Raval Ritu
Department of Biotechnology, Manipal Institute of Technology (MIT), Manipal Academy of Higher Education (MAHE), Manipal, 576104, Karnataka, India.
Manipal Biomachines, Manipal Institute of Technology (MIT), Manipal Academy of Higher Education (MAHE), Manipal, 576104, Karnataka, India.
Synth Syst Biotechnol. 2024 Dec 20;10(2):356-364. doi: 10.1016/j.synbio.2024.12.005. eCollection 2025 Jun.
As synthetic biology advances, the necessity for robust biocontainment strategies for genetically engineered organisms (GEOs) grows increasingly critical to mitigate biosafety risks related to their potential environmental release. This paper aims to evaluate environment signal-dependent biocontainment systems for engineered organisms, focusing specifically on leveraging triggered responses and combinatorial systems. There are different types of triggers-chemical, light, temperature, and pH-this review illustrates how these systems can be designed to respond to environmental signals, ensuring a higher safety profile. It also focuses on combinatorial biocontainment to avoid consequences of unintended GEO release into an external environment. Case studies are discussed to demonstrate the practical applications of these systems in real-world scenarios.
随着合成生物学的发展，为减轻与基因工程生物（GEOs）潜在环境释放相关的生物安全风险，对其采用强大的生物防护策略的必要性日益关键。本文旨在评估工程生物的环境信号依赖型生物防护系统，特别关注利用触发反应和组合系统。存在不同类型的触发因素——化学、光、温度和pH——本综述阐述了如何设计这些系统以响应环境信号，确保更高的安全性。它还关注组合生物防护，以避免基因工程生物意外释放到外部环境的后果。文中讨论了案例研究，以展示这些系统在实际场景中的实际应用。
