铁代谢相关基因和线粒体基因在小鼠乳腺退化过程中被诱导。

Iron metabolism-related genes and mitochondrial genes are induced during involution of mouse mammary gland.

作者信息

Lee M, Kim H, Jeon D, Hwang I, Choi B, Myung K, Kim H, Choi Y, Paik S, Baik M

机构信息

Department of Genetic Engineering, Chonnam National University, Kwangju, Korea.

出版信息

Biochem Biophys Res Commun. 1996 Jul 5;224(1):164-8. doi: 10.1006/bbrc.1996.1001.

DOI:10.1006/bbrc.1996.1001

PMID:8694805

Abstract

To understand molecular mechanisms of mammary gland involution, several clones were isolated after the primary differential screening of a total 40,000 pfu of involution-specific cDNA library, and further characterized. The partial sequences and Northern analysis revealed that iron metabolism-related genes and mitochondrial genes were induced during mammary gland involution. The expression of the lactoferrin gene was induced at involution days 1, 2, and 3. The expression of ferritin heavy chain gene was induced at involution days 1, 2, 3 and 4. Cytochrome oxidase subunit 1 and cytochrome oxidase subunit 2 genes were induced at involution days 4 and 7. The expression of cytochrome b gene was induced at involution day 7. These results imply that iron metabolism and mitochondrial function may be altered during mammary gland involution.

摘要

为了解乳腺退化的分子机制，在对总共40,000个噬菌斑形成单位的退化特异性cDNA文库进行初次差异筛选后，分离出了几个克隆，并对其进行了进一步表征。部分序列和Northern分析表明，铁代谢相关基因和线粒体基因在乳腺退化过程中被诱导表达。乳铁蛋白基因的表达在退化第1、2和3天被诱导。铁蛋白重链基因的表达在退化第1、2、3和4天被诱导。细胞色素氧化酶亚基1和细胞色素氧化酶亚基2基因在退化第4和7天被诱导。细胞色素b基因的表达在退化第7天被诱导。这些结果表明，在乳腺退化过程中铁代谢和线粒体功能可能会发生改变。