从嗜铬细胞瘤中分离人1型GTP环化水解酶的全长cDNA克隆。
Isolation of a full-length cDNA clone for human GTP cyclohydrolase I type 1 from pheochromocytoma.
作者信息
Nomura T, Ohtsuki M, Matsui S, Sumi-Ichinose C, Nomura H, Hagino Y, Iwase K, Ichinose H, Fujita K, Nagatsu T
机构信息
Department of Pharmacology, Fujita Health University School of Medicine, Aichi, Japan.
出版信息
J Neural Transm Gen Sect. 1995;101(1-3):237-42. doi: 10.1007/BF01271561.
Although the existence of three different cDNA forms of human GTP cyclohydrolase I (GCH I) have been reported (Togari et al., 1992), the full-length sequence of any human GCH I cDNA involving poly (A) tail has not yet been documented. In the present study, we first isolated a full-length cDNA clone encoding human GCH I type 1 from human pheochromocytoma cDNA library. The length of the cDNA insert was 2,921 base pairs including poly (A) tail. RNA blot analysis showed a single mRNA species of 4.0 kb in human pheochromocytoma tissue.
尽管已有报道称存在三种不同形式的人鸟苷三磷酸环化水解酶I（GCH I）的cDNA（Togari等人，1992年），但尚未记录任何包含聚腺苷酸尾的人GCH I cDNA的全长序列。在本研究中，我们首先从人嗜铬细胞瘤cDNA文库中分离出一个编码人1型GCH I的全长cDNA克隆。cDNA插入片段的长度为2921个碱基对，包括聚腺苷酸尾。RNA印迹分析显示人嗜铬细胞瘤组织中有一个4.0 kb的单一mRNA种类。
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