编码人芳香族L-氨基酸脱羧酶的cDNA克隆的分离与鉴定。

Isolation and characterization of a cDNA clone encoding human aromatic L-amino acid decarboxylase.

作者信息

Ichinose H, Kurosawa Y, Titani K, Fujita K, Nagatsu T

机构信息

Department of Biochemistry, Nagoya University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1989 Nov 15;164(3):1024-30. doi: 10.1016/0006-291x(89)91772-5.

DOI:10.1016/0006-291x(89)91772-5

PMID:2590185

Abstract

The nucleotide sequence of a cDNA clone that includes the entire coding region of human aromatic L-amino acid decarboxylase gene is presented. A human pheochromocytoma cDNA library was screened using an oligonucleotide probe which corresponded to a partial amino acid sequence of the enzyme purified from the human pheochromocytoma. The isolated cDNA clone encoded a protein of 480 amino acids with a calculated molecular mass of 53.9 kDa. The amino acid sequence Asn-Phe-Asn-Pro-His-Lys-Trp around a possible cofactor (pyridoxal phosphate) binding site is identical in human, Drosophila, and pig enzymes.

摘要

本文给出了一个包含人芳香族L-氨基酸脱羧酶基因完整编码区的cDNA克隆的核苷酸序列。使用与从人嗜铬细胞瘤中纯化的该酶部分氨基酸序列相对应的寡核苷酸探针筛选人嗜铬细胞瘤cDNA文库。分离得到的cDNA克隆编码一个由480个氨基酸组成的蛋白质，计算分子量为53.9 kDa。人、果蝇和猪的该酶在一个可能的辅因子（磷酸吡哆醛）结合位点周围的氨基酸序列Asn-Phe-Asn-Pro-His-Lys-Trp是相同的。