O'Driscoll L, Kennedy S, McDermott E, Kelehan P, Clynes M
National Cell & Tissue Culture Centre/BioResearch Ireland.
Eur J Cancer. 1996 Jan;32A(1):128-33. doi: 10.1016/0959-8049(95)00552-8.
A method is described by which RNA, suitable for reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, can be extracted from formalin-fixed paraffin-embedded (FFPE) tissues and subsequently used for detecting the expression of several genes. Using this technique, RNA can be extracted from specimens, quantified, reverse transcribed and regions of interest amplified and analysed within 36 h. The tissue specimens included in this study were from human breast carcinoma, investigating a range of genes associated with the development and/or maintenance of multiple drug resistance (MDR). This technique, applied to archival tissues, offers great potential for increasing our understanding of alterations in expression levels of genes associated with MDR. The method developed is also applicable to studies on expression of other genes in paraffin-embedded tissues.
本文描述了一种方法，通过该方法可从福尔马林固定石蜡包埋（FFPE）组织中提取适用于逆转录聚合酶链反应（RT-PCR）分析的RNA，随后用于检测多个基因的表达。使用该技术，可在36小时内从标本中提取RNA、进行定量、逆转录，并对感兴趣区域进行扩增和分析。本研究纳入的组织标本来自人乳腺癌，研究了一系列与多药耐药（MDR）的发生和/或维持相关的基因。该技术应用于存档组织，为增进我们对与MDR相关基因表达水平改变的理解提供了巨大潜力。所开发的方法也适用于石蜡包埋组织中其他基因表达的研究。
