Dehority B A, Grubb J A
Appl Environ Microbiol. 1977 Apr;33(4):998-1001. doi: 10.1128/aem.33.4.998-1001.1977.
When glucose-1-phosphate was used as the only added energy source in a selective roll tube medium, colony counts for rumen contents ranged from 17.8 to 84.8% of the total culturable count. Percentages were highest in rumen contents from sheep fed high-concentrate rations. From a total of 73 cultures isolated from glucose-1-phosphate roll rubes, only 15.1% were presumptively identified as Bacteroides species. Strains presumptively identified as Butyrivibrio, Selenomonas, Treponema, Streptococcus bovis, and Lachnospira also fermented glucose-1-phosphate. Thus, glucose-1-phosphate would not be useful as a selective substrate for isolation or enumeration of Bacteroides species from the rumen.
当在选择性滚管培养基中仅使用葡萄糖-1-磷酸作为添加的能源时，瘤胃内容物的菌落计数占可培养总数的17.8%至84.8%。在饲喂高浓缩日粮的绵羊瘤胃内容物中，该百分比最高。从葡萄糖-1-磷酸滚管中分离出的73种培养物中，只有15.1%被初步鉴定为拟杆菌属。初步鉴定为丁酸弧菌属、月形单胞菌属、密螺旋体属、牛链球菌和毛螺菌属的菌株也能发酵葡萄糖-1-磷酸。因此，葡萄糖-1-磷酸作为从瘤胃中分离或计数拟杆菌属的选择性底物并无用处。
