Motta C, Gueux E, Mazur A, Rayssiguier Y
Centre de Recherche en Nutrition Humaine de Clermont-Ferrand, Hôtel Dieu, Clermont-Ferrand, France.
Br J Nutr. 1996 May;75(5):767-73. doi: 10.1079/bjn19960180.
Triacylglycerol-rich lipoproteins (TGRLP) were isolated from Cu-deficient and control rats. TGRLP from Cu-deficient rats appeared more fluid than those from controls as sensed by the fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH). This high fluidity was related to a low cholesterol:phospholipid ratio and high triacylglycerol content in these lipoproteins. TGRLP from Cu-deficient rats were more susceptible to in vitro peroxidation than lipoproteins from control rats as shown by the rate of diene conjugation. The damage induced by the peroxidation resulted in a more ordered state of the lipid fraction especially in lipoproteins from Cu-deficient rats. Thus, after in vitro peroxidation, TGRLP from Cu-deficient rats were more rigid than those from controls. These results suggest that Cu deficiency induces modifications in physicochemical properties of TGRLP which could affect their metabolism.
从缺铜大鼠和对照大鼠中分离出富含三酰甘油的脂蛋白（TGRLP）。通过1,6-二苯基-1,3,5-己三烯（DPH）的荧光各向异性检测发现，缺铜大鼠的TGRLP比对照大鼠的TGRLP流动性更强。这种高流动性与这些脂蛋白中低胆固醇：磷脂比率和高三酰甘油含量有关。如二烯共轭速率所示，缺铜大鼠的TGRLP比对照大鼠的脂蛋白在体外更易发生过氧化。过氧化诱导的损伤导致脂质部分处于更有序的状态，尤其是缺铜大鼠的脂蛋白。因此，体外过氧化后，缺铜大鼠的TGRLP比对照大鼠的TGRLP更僵硬。这些结果表明，铜缺乏会引起TGRLP物理化学性质的改变，这可能会影响它们的代谢。
