Bussière L, Mazur A, Gueux E, Nowacki W, Rayssiguier Y
Laboratorie des Maladies Métaboliques, INRA, Theix, St Genès Champanelle, France.
Magnes Res. 1995 Jun;8(2):151-7.
An important characteristic of hyperlipaemia associated with magnesium deficiency in rats is the postprandial accumulation of triglyceride-rich lipoproteins (TGRLP). The present investigation was performed to determine the susceptibility of TGRLP isolated from magnesium-deficient rats to metal ion and cell dependent peroxidation and the effect of these lipoproteins on cultured vascular smooth muscle cells (VSMC). TGRLP were isolated by sequential ultracentrifugation from plasma of control and magnesium-deficient rats fed for 8 d on adequate or magnesium-deficient diets. Magnesium-deficient animals were hypertriglyceridemic as compared to control rats. After exposure to oxidative stress in vitro, Cu2+ or activated macrophages, conjugated diene production was significantly higher in TGRLP isolated from magnesium-deficient rats. Culture of VSMC with TGRLP from magnesium-deficient rats resulted in more important cell growth than with lipoproteins isolated from control animals. After incubation with VSMC, TGRLP from magnesium-deficient rats showed higher conjugated diene production than those from control rats. These results support the atherogenic properties of magnesium deficiency, which is accompanied by hyperlipaemia and which affects two important linked pathways in atherosclerosis, lipoprotein peroxidation and VSMC proliferation.
大鼠体内与镁缺乏相关的高脂血症的一个重要特征是餐后富含甘油三酯的脂蛋白（TGRLP）的积累。本研究旨在确定从缺镁大鼠中分离出的TGRLP对金属离子和细胞依赖性过氧化的敏感性，以及这些脂蛋白对培养的血管平滑肌细胞（VSMC）的影响。通过连续超速离心从喂食充足或缺镁饮食8天的对照大鼠和缺镁大鼠的血浆中分离TGRLP。与对照大鼠相比，缺镁动物具有高甘油三酯血症。在体外暴露于氧化应激、Cu2+或活化的巨噬细胞后，从缺镁大鼠中分离出的TGRLP中共轭二烯的产生明显更高。用来自缺镁大鼠的TGRLP培养VSMC比用来自对照动物的脂蛋白培养导致更显著的细胞生长。与VSMC孵育后，来自缺镁大鼠的TGRLP显示出比来自对照大鼠的TGRLP更高的共轭二烯产生。这些结果支持了镁缺乏的致动脉粥样硬化特性，镁缺乏伴有高脂血症，并影响动脉粥样硬化中两个重要的相关途径，即脂蛋白过氧化和VSMC增殖。
