溶藻弧菌编码Na⁺/H⁺逆向转运蛋白的nhaB基因的克隆与测序
Cloning and sequencing of nhaB gene encoding an Na+/H+ antiporter from Vibrio alginolyticus.
作者信息
Nakamura T, Enomoto H, Unemoto T
机构信息
Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
出版信息
Biochim Biophys Acta. 1996 Jul 31;1275(3):157-60. doi: 10.1016/0005-2728(96)00034-5.
A gene has been cloned from the marine bacterium Vibrio alginolyticus that functionally complements a mutant strain of Escherichia coli, TO114, defective in three Na+/H+ antiport genes (nhaA, nhaB, chaA). The nucleotide sequence of the cloned fragment revealed an open reading frame, which encodes a protein with a predicted 528 amino acid sequence and molecular mass of 57212 Da. This gene has 62% identity to nhaB gene at the DNA level from Escherichia coli and the deduced amino acid sequence is 67% identical with E. coli NhaB. This gene is presumably the V. alginolyticus nhaB gene and will be named nhaBv.
已从海洋溶藻弧菌中克隆出一个基因，该基因可在功能上互补大肠杆菌TO114的一个突变菌株，该突变菌株在三个Na⁺/H⁺逆向转运蛋白基因（nhaA、nhaB、chaA）上存在缺陷。克隆片段的核苷酸序列揭示了一个开放阅读框，其编码一种预测有528个氨基酸序列、分子量为57212 Da的蛋白质。该基因在DNA水平上与大肠杆菌的nhaB基因有62%的同一性，推导的氨基酸序列与大肠杆菌NhaB有67%的同一性。这个基因推测是溶藻弧菌的nhaB基因，将被命名为nhaBv。
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