Cao X, Zhang W, Tao Q
Department of Immunology, Second Military Medical University, Shanghai.
Zhonghua Yi Xue Za Zhi. 1995 Oct;75(10):602-5, 639.
It has been shown that IL-2 and IL-6 can cooperate to induce immune responses more effectively. In order to increase the therapeutic efficacy of cytokine gene-transfected tumor vaccines, we treated the experimental pulmonary metastatic melanoma-bearing mice with IL-2 gene-transfected tumor vaccine and IL-6 gene-transfected tumor vaccine. After the combined treatment, the pulmonary metastases were reduced more significantly and the survival time of tumor-bearing mice was also prolonged more significantly. The CTL activity, NK activity and IL-2-induced LAK activity, IL-2 and TNF secretion from the splenocytes of the above tumor-bearing mice increased more significantly than that of tumor-bearing mice treated with IL-2 gene-transfected vaccine or IL-6 gene-transfected vaccine alone. These results demonstrated that the combined use of IL-2 gene-transfected tumor vaccine and IL-6 gene-transfected tumor vaccine could achieve more potent antitumor effect via more efficient activation of immune functions. The experiment outlines a novel approach to the cytokine gene therapy of cancer.
已表明白细胞介素-2（IL-2）和白细胞介素-6（IL-6）可协同作用更有效地诱导免疫反应。为提高细胞因子基因转染肿瘤疫苗的治疗效果，我们用IL-2基因转染肿瘤疫苗和IL-6基因转染肿瘤疫苗处理实验性肺转移黑色素瘤荷瘤小鼠。联合治疗后，肺转移明显减少，荷瘤小鼠的生存时间也显著延长。上述荷瘤小鼠脾细胞的细胞毒性T淋巴细胞（CTL）活性、自然杀伤（NK）活性以及IL-2诱导的淋巴因子激活的杀伤细胞（LAK）活性、IL-2和肿瘤坏死因子（TNF）分泌比单独用IL-2基因转染疫苗或IL-6基因转染疫苗处理的荷瘤小鼠增加更显著。这些结果表明，联合使用IL-2基因转染肿瘤疫苗和IL-6基因转染肿瘤疫苗可通过更有效地激活免疫功能实现更强的抗肿瘤作用。该实验概述了一种癌症细胞因子基因治疗的新方法。
