Cao X, Zhang W, Wan T, Yu Y, Tao Q, Wang J
Department of Immunology, Second Military Medical University, Shanghai, P.R. China.
J Exp Clin Cancer Res. 1999 Jun;18(2):173-9.
To enhance the antitumor immunity induced by IL-2 gene-modified tumor vaccine, we proposed a combined protocol to treat tumor-bearing mice using IL-2 gene-modified tumor vaccine in combination with IL-1 and low-dose Cyclophosphamide(Cy). After treatment with IL-2 gene-modified B16 melanoma cell vaccine alone, the pulmonary metastases of tumor-bearing mice were reduced and their survival time was prolonged. The anti-metastases effect was improved when the vaccine was used in combination with IL-1 or low-dose Cy. The best therapeutic effect was achieved when the IL-2 gene-modified vaccine was combined with IL-1 and low-dose Cy. The cytotoxicity of the splenic CTL, NK, and the levels of IL-2, TNF secreted by splenocytes increased after tumor-bearing mice were treated with the IL-2 gene-modified tumor vaccine. The above antitumor immune functions were augmented more significantly when IL-1, low-dose Cy were used in combination with IL-2 genemodified tumor vaccine. These results demonstrated that the IL-2 gene modified vaccine could exert more potent anti-metastases effects when it is combined with IL-1 or/and low-dose Cy by activating the specific and non-specific antitumor immune responses more effectively.
为增强白细胞介素-2（IL-2）基因修饰的肿瘤疫苗诱导的抗肿瘤免疫，我们提出了一种联合方案，使用IL-2基因修饰的肿瘤疫苗联合IL-1和低剂量环磷酰胺（Cy）治疗荷瘤小鼠。单独使用IL-2基因修饰的B16黑色素瘤细胞疫苗治疗后，荷瘤小鼠的肺转移减少，生存时间延长。当疫苗与IL-1或低剂量Cy联合使用时，抗转移效果得到改善。当IL-2基因修饰的疫苗与IL-1和低剂量Cy联合使用时，可获得最佳治疗效果。荷瘤小鼠经IL-2基因修饰的肿瘤疫苗治疗后，脾细胞毒性T淋巴细胞（CTL）、自然杀伤细胞（NK）的细胞毒性以及脾细胞分泌的IL-2、肿瘤坏死因子（TNF）水平均升高。当IL-1、低剂量Cy与IL-2基因修饰的肿瘤疫苗联合使用时，上述抗肿瘤免疫功能增强更为显著。这些结果表明，IL-2基因修饰的疫苗与IL-1或/和低剂量Cy联合使用时，通过更有效地激活特异性和非特异性抗肿瘤免疫反应，可发挥更强的抗转移作用。
