Li B, Zhang X, Cai Y
National Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Yi Xue Za Zhi. 1995 Nov;75(11):683-5, 711.
All-trans-retinoic acid (RA) is a powerful differentiation-inducing reagent. We examined the effect of RA on malignant phenotype of human lung adenocarcinoma cell line GLC-82 in vitro. Treatment of GLC-82 cell with 10(-5)mol/L. RA for 1-7 days resulted in suppression of cell proliferation (33-55%), inhibition of colony formation in soft agar (97.5%), and a decrease of 3H-TdR incorporation (30-60%). Cytokinetic studies demonstrated that the cells arrested in G1/G0 phase increased from 36.0% to 72.4%, which is typical for cell differentiation. Human endothelial cell transglytaminase (TGase) was expressed persistently during RA treatment. Treatment of GLC-82 cell with RA gave rise to senescence and apoptosis gradually. The results indicated that induction of differentiation and modulation of gene expression can be achieved by RA treatment in human lung adenocarcinoma cell line GLC-82.
全反式维甲酸（RA）是一种强大的分化诱导剂。我们在体外研究了RA对人肺腺癌细胞系GLC - 82恶性表型的影响。用10^(-5)mol/L的RA处理GLC - 82细胞1 - 7天，导致细胞增殖受到抑制（33 - 55%），软琼脂中集落形成受到抑制（97.5%），并且3H - TdR掺入量减少（30 - 60%）。细胞动力学研究表明，停滞在G1/G0期的细胞从36.0%增加到72.4%，这是细胞分化的典型特征。人内皮细胞转谷氨酰胺酶（TGase）在RA处理期间持续表达。用RA处理GLC - 82细胞会逐渐导致细胞衰老和凋亡。结果表明，通过RA处理人肺腺癌细胞系GLC - 82可以实现分化诱导和基因表达调控。
