Ai Z W
Shanghai Medical College.
Zhonghua Zhong Liu Za Zhi. 1991 Jan;13(1):9-12.
Reversing effect of retinoic acid (RA) on some phenotypes of human hepatocarcinoma cell line was studied. It was found that the growth of SMMC-7721 human hepatocarcinoma cell line was markedly inhibited when cultured in 10 mumol/L RA. Morphology of cells treated with RA reversed to normal when observed under light and electron microscopes and by image analysis. The 3H-TdR incorporation declined. By flow cytometry, it was observed that cells in G0/G1 phase increased from 49.6% to 59.1%, whereas cells in S or G2 + M phase decreased from 50.4% to 40.9% after 3-day RA treatment. The major distribution of chromosomes changed from 48-56 to 44-50 with an increase in diploid (from 4% to 15%). The results indicate that RA could reverse some phenotypes of human hepatocarcinoma cell line.
研究了维甲酸（RA）对人肝癌细胞系某些表型的逆转作用。发现当在10μmol/L RA中培养时，SMMC - 7721人肝癌细胞系的生长受到明显抑制。用光镜、电镜及图像分析观察，经RA处理的细胞形态恢复正常。3H - TdR掺入量下降。通过流式细胞术观察到，经3天RA处理后，G0/G1期细胞从49.6%增加到59.1%，而S期或G2 + M期细胞从50.4%下降到40.9%。染色体的主要分布从48 - 56变为44 - 50，二倍体增加（从4%增至15%）。结果表明，RA可逆转人肝癌细胞系的某些表型。
