Traub W H, Bauer D, Leonhard B
Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg/Saar, Germany.
Chemotherapy. 1996 Mar-Apr;42(2):118-32. doi: 10.1159/000239431.
Teichoic acid (TA) and peptidoglycan (PG) extracted from Staphylococcus aureus strains ATCC 25923 and Lafferty as well as formalinized cells of these two strains and several clinical methicillin-resistant S. aureus (MRSA) isolates were immunogenic for New Zealand White rabbits. Rabbits which had recovered from experimental bacteremia due to MRSA seroconverted, i.e. demonstrated raised titers of antibodies against TA and PG of the S. aureus strain Lafferty and against whole cells (WC) and ultrasound cell lysates (UCL) of MRSA isolates No. 1 and 2 (representative of nosocomial MRSA strain I), as determined with enzyme-linked immunosorbent assays. Furthermore, sera from 2 long-term survivor rabbits recognized four polypeptides (apparent molecular weight = 38.9, 33.9, 30.9, and 28.2 kDa) shared by UCL extracts from MRSA isolates No. 1 and 2, as determined with immunoblots. Neither normal nor immune rabbit sera augmented the bactericidal activity of fresh defibrinated human blood (65% v/v) against selected MRSA isolates and S. aureus strain ATCC 25923. Sera from 12 patients with documented systemic infection due to MRSA outbreak strain I were examined for IgM and IgG antibodies against TA, WC, and UCL antigens. Three patient sera exhibited raised IgM antibodies against TA; 7 of 12 patient sera showed increased IgG anti-TA titers. Only 1 patient had a markedly raised IgM anti-WC titer, whereas 4 and 10 of the patients had increased IgG titers against WC from MRSA isolates No. 1 and 2, respectively. However, all 12 patients had raised IgG titers against UCL from MRSA isolate No.2 versus 4 of 12 patients with elevated IgG titers against UCL from MRSA isolate No.1. Immunoblots with 3 selected patient sera revealed IgG antibodies to be more multifaceted than IgM antibodies. Sera from 11 of the 12 patients contained antimicrobial drug(s); yet only 5 of these 11 sera (used at 10% v/v in broth) killed inocula of MRSA isolate No. 43. None of the 12 patient sera (10% v/v) enhanced the bactericidal activity of human blood against selected MRSA isolates. Neither three commercial intravenously applicable IgG preparations nor an IgG anti-alpha-hemolysin formulation (employed at 10% v/v) augmented the bactericidal activity of fresh defibrinated human blood against selected MRSA isolates comprising MRSA outbreak strain I.
从金黄色葡萄球菌菌株ATCC 25923和Lafferty中提取的磷壁酸（TA）和肽聚糖（PG），以及这两种菌株的甲醛固定化细胞和几株临床耐甲氧西林金黄色葡萄球菌（MRSA）分离株，对新西兰白兔具有免疫原性。因MRSA导致实验性菌血症后康复的兔子发生了血清转化，即通过酶联免疫吸附测定法测定，其针对金黄色葡萄球菌菌株Lafferty的TA和PG以及针对MRSA分离株1号和2号（代表医院内MRSA菌株I）的全细胞（WC）和超声细胞裂解物（UCL）的抗体滴度升高。此外，用免疫印迹法测定，2只长期存活兔子的血清识别出MRSA分离株1号和2号的UCL提取物共有的4种多肽（表观分子量 = 38.9、33.9、30.9和28.2 kDa）。正常兔血清和免疫兔血清均未增强新鲜去纤维蛋白人血（65% v/v）对选定的MRSA分离株和金黄色葡萄球菌菌株ATCC 25923的杀菌活性。检测了12例因MRSA暴发菌株I导致有记录的全身感染患者的血清中针对TA、WC和UCL抗原的IgM和IgG抗体。3例患者血清中针对TA的IgM抗体升高；12例患者血清中有7例显示抗TA IgG滴度升高。只有1例患者的抗WC IgM滴度明显升高，而分别有4例和10例患者针对MRSA分离株1号和2号的WC的IgG滴度升高。然而，所有12例患者针对MRSA分离株No.2的UCL的IgG滴度均升高，而针对MRSA分离株No.1的UCL的IgG滴度升高的患者有4例。用3份选定患者血清进行的免疫印迹显示，IgG抗体比IgM抗体更具多样性。12例患者中有11例的血清含有抗菌药物；但这11份血清中只有5份（在肉汤中以10% v/v使用）能杀死MRSA分离株No.43的接种物。12例患者血清（10% v/v）均未增强人血对选定的MRSA分离株的杀菌活性。3种市售静脉适用的IgG制剂和1种抗α-溶血素IgG制剂（以10% v/v使用）均未增强新鲜去纤维蛋白人血对包括MRSA暴发菌株I在内的选定MRSA分离株的杀菌活性。
