Yuan S, Huang G, Wang X
Institute of Cancer Research, West China University of Medical Sciences, Chengdu.
Zhonghua Zhong Liu Za Zhi. 1995 Nov;17(6):422-4.
The differentiation-inducing activity of tanshinone (TAN) and all-trans-retinoic acid (RA) was studied in vitro on a human cervical carcinoma cell line, ME180. The tumor cells were treated with TAN or RA in DMSO (final concentration 0.02%, V/V) on 4 successive days. Cells treated with the same concentration of DMSO alone served as control. Morphologic studies with light and transmission electron microscopy showed that the cells treated with both TAN and RA became well-differentiated. The cell growth, (as revealed by cell counting and 3H-TdR incorporation) was inhibited and the tumorigenicity in nude mice was reduced. No significant difference was observed between the cells treated with TAN and RA.
在体外研究了丹参酮（TAN）和全反式维甲酸（RA）对人宫颈癌细胞系ME180的诱导分化活性。肿瘤细胞连续4天用溶于二甲基亚砜（DMSO，终浓度0.02%，V/V）的TAN或RA处理。仅用相同浓度DMSO处理的细胞作为对照。光镜和透射电镜的形态学研究表明，用TAN和RA处理的细胞均出现了良好的分化。细胞生长（通过细胞计数和3H-胸腺嘧啶核苷掺入显示）受到抑制，并且在裸鼠中的致瘤性降低。在用TAN和RA处理的细胞之间未观察到显著差异。
