Sun Y X, He Z P, Chen S S
Shanghai Second University of Medical Science.
Zhonghua Zhong Liu Za Zhi. 1994 Jan;16(1):15-7.
The growth rate and weight of transplanted tumor of SGC7901 gastric cancer cell line, treated with 10 mumol/L retinoic acid (RA) in nude mice, were significantly reduced in comparison with those of control cells. The morphology of RA-treated cells was changed from ellipse shape with meandering margin of a large nucleus to shuttle shape with smooth margin of a small nucleus. With flow cytometry, it was found that G/O Phase markedly increased after the RA treatment, whereas S and G2 + M phase decreased. Especially the S phase fraction was lower than that of control cells. The above results indicated that RA could reverse biological and morphological phenotypes of the human gastric cancer cells in vitro and could induce differentiation of gastric cancer cells to a certain extent.
在裸鼠中，用10μmol/L视黄酸（RA）处理SGC7901胃癌细胞系移植瘤，其生长速率和重量与对照细胞相比显著降低。经RA处理的细胞形态从具有大核且边缘曲折的椭圆形变为具有小核且边缘光滑的梭形。通过流式细胞术发现，RA处理后G/O期明显增加，而S期和G2+M期减少。特别是S期比例低于对照细胞。上述结果表明，RA可在体外逆转人胃癌细胞的生物学和形态学表型，并能在一定程度上诱导胃癌细胞分化。
