Ichikawa N, Ishida F, Shimodaira S, Tahara T, Kato T, Kitano K
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Thromb Haemost. 1996 Aug;76(2):156-60.
To clarify the regulatory mechanism of thrombopoietin (TPO, c-Mpl ligand) in chronic thrombocytopenic conditions, we determined TPO levels in the sera of patients with aplastic anaemia (AA; n = 26) and idiopathic thrombocytopenic purpura (ITP; n = 32) by an enzyme-linked immunosorbent assay. Despite a similarity in platelet counts, serum TPO levels in the AA group were markedly higher than those in the ITP group: 20.41 +/- 9.71 f mol/ml (mean +/- SD) and 1.66 +/- 0.55 f mol/ml, respectively, both of which were significantly elevated compared to normal subjects (n = 41; 1.22 +/- 0.37). In both groups, serum TPO level showed an inverse correlation with the platelet count. We determined the megakaryocyte volume using bone marrow clot section and found that it was markedly small in the AA group; while in the ITP group it was augmented with a correlation to serum TPO level. Our findings suggest that TPO levels may be regulated not only by platelets but also megakaryocytes in AA and ITP.
为阐明血小板生成素（TPO，c-Mpl配体）在慢性血小板减少症中的调节机制，我们采用酶联免疫吸附测定法测定了再生障碍性贫血（AA；n = 26）和特发性血小板减少性紫癜（ITP；n = 32）患者血清中的TPO水平。尽管两组患者的血小板计数相似，但AA组的血清TPO水平显著高于ITP组：分别为20.41±9.71 fmol/ml（平均值±标准差）和1.66±0.55 fmol/ml，与正常受试者（n = 41；1.22±0.37）相比均显著升高。在两组中，血清TPO水平与血小板计数呈负相关。我们使用骨髓凝块切片测定巨核细胞体积，发现AA组明显较小；而ITP组则增大，且与血清TPO水平相关。我们的研究结果表明，在AA和ITP中，TPO水平可能不仅受血小板调节，还受巨核细胞调节。
