Thompson F M, Mayrhofer G, Cummins A G
Gastroenterology Unit, The Queen Elizabeth Hospital, Woodville South, South Australia, Australia.
Gastroenterology. 1996 Jul;111(1):37-44. doi: 10.1053/gast.1996.v111.pm8698223.
BACKGROUND & AIMS: Intestinal crypt hyperplasia is associated with local T-cell activation in both clinical and experimental examples of immunologically mediated enteropathy. This suggests that T cell-derived factors may be trophic for epithelial proliferation in the intestine postnatally. The purpose of this study was to investigate T-cell activity during weaning in the rat and to investigate immune dependence of intestinal growth on T-cell activation.
The expression of interleukin-2 receptor (IL-2R) by mesenteric lymph node T cells was investigated from days 14 to 160 of life. Rats were treated with monoclonal antibodies against the IL-2R that were nonblocking (control) or blocking (experimental) from day 7, and intestinal growth was assessed at days 19, 25, and 29 of life.
The mean +/- SEM of T cells expressing the IL-2R during weaning (days 15-28) was 6.1% +/- 0.3% compared with 3.3% +/- 0.3% at other ages (P < 0.001). The small intestine in rats treated with blocking antibody had reduced crypt length and mitotic count compared with control animals.
Weaning is associated with activation of T cells and blockade of the IL-2R reduces intestinal growth.
在免疫介导性肠病的临床及实验实例中，肠道隐窝增生与局部T细胞活化相关。这表明T细胞衍生因子可能在出生后对肠道上皮增殖具有营养作用。本研究的目的是调查大鼠断奶期间的T细胞活性，并研究肠道生长对T细胞活化的免疫依赖性。
在出生后第14天至160天，研究肠系膜淋巴结T细胞白细胞介素-2受体（IL-2R）的表达。从第7天开始，用非阻断性（对照）或阻断性（实验）抗IL-2R单克隆抗体处理大鼠，并在出生后第19天、25天和29天评估肠道生长情况。
断奶期间（第15 - 28天）表达IL-2R的T细胞的平均±标准误为6.1%±0.3%，而其他年龄段为3.3%±0.3%（P < 0.001）。与对照动物相比，用阻断抗体处理的大鼠小肠隐窝长度和有丝分裂计数减少。
断奶与T细胞活化相关，阻断IL-2R会降低肠道生长。
