编码hnRNP I/PTB蛋白的人类基因在染色体19p13.3和14q23上的双重定位。

Dual localization of the human gene encoding hnRNP I/PTB protein to chromosomes 19p13.3 and 14q23.

作者信息

Della Valle V, le Coniat M, Soulard M, Nguyen V C, Berger R, Larsen C J

机构信息

INSERM U-301, Paris, France.

出版信息

Hum Genet. 1996 Aug;98(2):210-3. doi: 10.1007/s004390050193.

DOI:10.1007/s004390050193

PMID:8698345

Abstract

A cDNA probe representative of the human hnRNP I/PTB gene was used to perform fluorescence in situ hybridization (FISH) on metaphases of human chromosomes. A new localization was found on band 19p13.3 in addition to the previously reported localization to band 14q23. Identical results were obtained when FISH analysis was repeated with probes covering different parts of the hnRNP I cDNA clone. This supported the notion that most, if not all, of the sequences of the different parts of this clone are present on both chromosomes. Moreover, Southern blot analysis of DNAs from interspecies somatic hybrids containing chromosomes 19 and 14 revealed that the whole hnRNP I cDNA probe generated very similar patterns in each hybrid DNA. These data suggest that two closely related copies of the hnRNP I gene exist in the human genome.

摘要

使用代表人类hnRNP I/PTB基因的cDNA探针，对人类染色体中期相进行荧光原位杂交（FISH）。除先前报道的定位于14q23带外，还在19p13.3带发现了一个新的定位。当用覆盖hnRNP I cDNA克隆不同部分的探针重复FISH分析时，得到了相同的结果。这支持了这样一种观点，即该克隆不同部分的大部分（如果不是全部）序列在两条染色体上均存在。此外，对含有19号和14号染色体的种间体细胞杂种的DNA进行Southern印迹分析表明，整个hnRNP I cDNA探针在每个杂种DNA中产生非常相似的模式。这些数据表明，人类基因组中存在两个密切相关的hnRNP I基因拷贝。