Kanegane H, Kasahara Y, Niida Y, Yachie A, Sughii S, Takatsu K, Taniguchi N, Miyawaki T
Department of Pediatrics, School of Medicine, Kanazawa University, Japan.
Immunology. 1996 Feb;87(2):186-90. doi: 10.1046/j.1365-2567.1996.446530.x.
Human memory (CD45RO+) CD4+ T cells can be distinguished into two subpopulations on the basis of expression of the lymph node homing receptor, L-selectin (CD62L). In a prior study we showed that human L-selectin-positive memory T-helper (Th) cells promote the maturation of IgG- and IgA-producing cells by naive B cells. To further elucidate the contribution of memory CD4+ T cells to B-cell differentiation, human memory CD4+ T cells with or without L-selectin expression were evaluated for production of cytokines that participate in regulation of immunoglobulin production. It was found that L-selectin-positive human memory CD4+ T cells produce mainly interleukin (IL)-4 and IL-5, whereas L-selectin-negative CD4+ T cells produce mainly interferon-gamma (IFN-gamma). This profile of cytokine expression coincides with the profile that distinguishes Th1 and Th2 subsets. In contrast to the murine system, IL-10 production was similarly contributed by human L-selectin-positive and -negative memory CD4+ T-cell subpopulations. These results suggest that the human L-selectin-negative and -positive subpopulations of human memory CD4+ T cells contain Th1-like and Th2-like cytokine-producing cells, respectively.
人类记忆性（CD45RO +）CD4 + T细胞可根据淋巴结归巢受体L-选择素（CD62L）的表达分为两个亚群。在先前的一项研究中，我们发现人类L-选择素阳性记忆性辅助性T（Th）细胞可促进幼稚B细胞向产生IgG和IgA的细胞成熟。为了进一步阐明记忆性CD4 + T细胞对B细胞分化的作用，我们评估了有或没有L-选择素表达的人类记忆性CD4 + T细胞产生参与免疫球蛋白产生调节的细胞因子的情况。结果发现，L-选择素阳性的人类记忆性CD4 + T细胞主要产生白细胞介素（IL）-4和IL-5，而L-选择素阴性的CD4 + T细胞主要产生干扰素-γ（IFN-γ）。这种细胞因子表达谱与区分Th1和Th2亚群的谱一致。与小鼠系统不同，人类L-选择素阳性和阴性记忆性CD4 + T细胞亚群对IL-10的产生贡献相似。这些结果表明，人类记忆性CD4 + T细胞的L-选择素阴性和阳性亚群分别包含产生Th1样和Th2样细胞因子的细胞。
