Longhurst C, Ehrenstein M R, Leaker B, Stevenson F K, Spellerberg M, Chapman C, Latchmen D, Isenberg D A, Cambridge G
Department of Medicine, Institute of Urology, Middlesex Hospital, London, UK.
Immunology. 1996 Feb;87(2):334-8. doi: 10.1046/j.1365-2567.1996.463529.x.
Circulating antibodies to myeloperoxidase (MPO) are associated primarily with pauci-immune glomerulonephritis and systemic vasculitis. Anti-MPO antibodies belong to a group of autoantibodies, anti-neutrophil cytoplasmic antibodies, that may play a pathogenic role in vasculitis. We have generated a human monoclonal anti-MPO antibody (E3-MPO) using peripheral blood lymphocytes from a patient with microscopic polyarteritis. Variable region gene analysis of E3-MPO showed that the VH region had 90% homology with the germ line gene VH4-21. E3-MPO was also shown to carry the 9G4 idiotope, which so far has been associated only with human antibodies that utilize the VH4-21 gene. The 9G4 idiotope was also expressed on anti-MPO antibodies in sera from the donor patient and from 4/7 additional patients with active, untreated vasculitis. The nucleotide sequences of both the variable heavy and light chains of E3-MPO showed evidence of an antigen-driven response.
抗髓过氧化物酶（MPO）循环抗体主要与寡免疫性肾小球肾炎和系统性血管炎相关。抗MPO抗体属于自身抗体——抗中性粒细胞胞浆抗体的一种，可能在血管炎中起致病作用。我们利用一名显微镜下多血管炎患者的外周血淋巴细胞制备了一种人源单克隆抗MPO抗体（E3-MPO）。对E3-MPO的可变区基因分析表明，VH区与种系基因VH4-21有90%的同源性。E3-MPO还被证明携带9G4独特型，迄今为止，该独特型仅与利用VH4-21基因的人抗体相关。供体患者以及另外7名活动性、未经治疗的血管炎患者中4人的血清抗MPO抗体也表达了9G4独特型。E3-MPO重链和轻链可变区的核苷酸序列均显示出抗原驱动反应的证据。
