Luini W, De Rossi M, Licciardello L, Colotta F, Mantovani A
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Int J Immunopharmacol. 1991;13(6):631-7. doi: 10.1016/0192-0561(91)90175-7.
The present study was designed to investigate the effect of membrane proteoglycans (MPG) from Klebsiella pneumoniae on production of the chemotactic cytokine, IL-8, and monocyte chemotactic protein (MCP) by human peripheral blood monocytes. Exposure of human peripheral blood monocytes to MPG in vitro induced high levels of mRNA transcripts for IL-8 and MCP, as assessed by Northern blot analysis. Cytokine gene expression was associated with the production of chemotactic activity in the supernatants. The levels of IL-8 and MCP expression induced by MPG were comparable with those elicited by LPS. Induction of chemotactic cytokines in mononuclear phagocytes may play a role in the immunomodulatory activity of MPG.
本研究旨在探讨肺炎克雷伯菌的膜蛋白聚糖（MPG）对人外周血单核细胞趋化细胞因子白细胞介素-8（IL-8）和单核细胞趋化蛋白（MCP）产生的影响。通过Northern印迹分析评估，体外将人外周血单核细胞暴露于MPG可诱导IL-8和MCP的高水平mRNA转录本。细胞因子基因表达与上清液中趋化活性的产生相关。MPG诱导的IL-8和MCP表达水平与脂多糖（LPS）诱导的水平相当。单核吞噬细胞中趋化细胞因子的诱导可能在MPG的免疫调节活性中起作用。
