Park J W, Ma S N, Song E S, Song C H, Chae M R, Park B H, Rho R W, Park S D, Kim H R
Department of Biochemistry and Anatomy, School of Medicine, Chonbuk National University, Chonju, Republic of Korea.
Infect Immun. 1996 Jul;64(7):2873-6. doi: 10.1128/iai.64.7.2873-2876.1996.
Vibrio vulnificus is an estuarine bacterium that causes septicemia and serious wound infection. Cytolysin produced by V. vulnificus has been incriminated as one of the important virulence determinants of bacterial infection. Cytolysin (8 hemolytic units) given intravenously to mice via their tail veins caused severe hemoconcentration and lethality. Cytolysin treatment greatly increased pulmonary wet weight and vascular permeability as measured by (125)I-labeled albumin leakage without affecting those factors of other organs significantly. Blood neutrophils were markedly decreased in number after cytolysin injection, with a concomitant increase in the level of pulmonary myeloperoxidase activity, indicating that cytolysin-induced neutropenia might be due to pulmonary sequestration of neutrophils. By microscopic examination, severe perivascular edema and neutrophil infiltration were evident in lung tissues. These results suggest that increased vascular permeability and neutrophil sequestration in the lungs are important factors in lethal activity by cytolysin.
创伤弧菌是一种可导致败血症和严重伤口感染的河口细菌。创伤弧菌产生的溶细胞素被认为是细菌感染的重要毒力决定因素之一。通过小鼠尾静脉向其静脉注射溶细胞素（8个溶血单位）会导致严重的血液浓缩和致死性。通过（125）I标记白蛋白渗漏测量，溶细胞素处理显著增加了肺湿重和血管通透性，而对其他器官的这些因素没有显著影响。注射溶细胞素后，血液中的中性粒细胞数量明显减少，同时肺髓过氧化物酶活性水平升高，这表明溶细胞素诱导的中性粒细胞减少可能是由于中性粒细胞在肺部的滞留。通过显微镜检查，肺组织中明显可见严重的血管周围水肿和中性粒细胞浸润。这些结果表明，肺部血管通透性增加和中性粒细胞滞留是溶细胞素致死活性的重要因素。
